小鼠骨髓细胞体外扩增及其造血重建的研究  

Studies on expansion ex vivo of murine bone marrow cells and its hematopoietic reconstitution capacity

作　　者：韩明哲[1] 万岁桂[1] 钱林生[1] 韩俊领[1] 李新[1] 赵英新[1] 彭勤兴[1] 何一心[1] 卞寿庚[1] 严文伟[1] 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所

出　　处：《中华血液学杂志》1997年第8期413-417,共5页Chinese Journal of Hematology

基　　金：国家教委霍英东基金;国家教委回国人员科研基金

摘　　要：目的：观察干细胞因子（ＳＣＦ）与白细胞介素（ＩＬ）－１或ＩＬ－３联合对经５氟尿嘧啶（５ＦＵ）处理的骨髓细胞的体外扩增作用和体外扩增细胞移植的小鼠造血恢复能力。方法：在含有细胞因子的液体培养体系中培养５ＦＵ处理的骨髓细胞，观察粒－巨噬细胞集落形成单位（ＣＦＵ－ＧＭ）和高增殖潜能集落形成细胞（ＨＰＰ－ＣＦＣ）数量的增加以及移植扩增细胞的小鼠外周血细胞恢复。结果：单用ＳＣＦ无扩增作用，但ＳＣＦ与ＩＬ－１或ＩＬ－３联合时，与对照组相比较ＣＦＵ－ＧＭ分别增加３３．７±１８．１倍和１８．１±６．３倍。ＨＰＰ－ＣＦＣ分别扩增１７．８±１０．５倍和１２．７±９．１倍。移植体外扩增细胞组与对照组比较，外周血细胞恢复时间缩短１～３天，并且生存率也显著提高。结论：在液体培养体系中ＳＣＦ与ＩＬ－１或ＩＬ－３联合对造血细胞扩增有协同作用，并且体外扩增后的骨髓细胞能加速移植受体的早期造血重建。Objective:To investigate the effects of stem cell factor (SCF) in combination with interleukin 1(IL 1) or/and interleukin 3(IL 3) on ex vivo expansion of 5FU treated bone marrow cells and hematopoietic recovery in lethally irradiated mice transplanted with the expanded cells.Methods:5FU treated bone marrow cells (d3 5FU BMC) were cultured in a cytokines containing medium,and the net increments of CFU GM and high proliferative potential colony forming cell (HPP CFC) were evaluated.Results: CFU GM increased by 33.7±18.1 or 18.1±6.3 fold, and HPP CFC by 17.8±10.5 or 12.7±9.1 fold, respectively,in cultures containing SCF with IL 1 or IL 3, as compared with that in control;while SCF alone had little effect.Compared with fresh d3 5FU BMC,transplantation of the expanded bone marrow cells accelerated the recovery of recipients' peripheral blood cell counts by 1～3 days and increased the survival rate of the transplanted animals (d3 5FU BMC group 50% vs expansion group 80%～100%).Conclusion:SCF in combination with IL 1 or IL 3 synergetically ex vivo expands hematopoietic cells.Transplantation of the expanded bone marrow cells accelerates the recipient's hematopoietic reconstitution.

关 键 词：扩增 造血重建 骨髓移植 造血干细胞移植 

分 类 号：R457.7[医药卫生—治疗学]