孕激素对绒毛外滋养细胞VEGF表达的影响  

The role of progesterone effect on VEGF expressed by extravillous trophoblast in the pregnancy

作　　者：李丽丹[1] 冯玲[1] 王少帅[1] 

机构地区：[1]华中科技大学附属同济医院妇产科,湖北武汉430030

出　　处：《中国优生与遗传杂志》2007年第12期29-30,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的通过研究孕激素对绒毛外滋养细胞孕激素受体(PR)和血管内皮生长因子表达的影响,探讨P对绒毛外滋养细胞侵袭能力的影响及维持妊娠的机理。方法采用半定量PCR和定量PCR测定孕激素干预后绒毛外滋养细胞表达PR mRNA和VEGF mRNA的水平。结果(1)不同浓度的孕激素PRA表达量极低,PRB mRNA的表达没有显著差异(P>0.05);(2)VEGF mRNA与孕激素浓度变化无明显相关,反而与PRB mRNA的水平成反比关系。结论(1)孕激素作用的发挥取决于PR的水平,与激素本身的浓度没有明显关系;(2)PRB能下调滋养细胞分泌VEGF的能力从而抑制其侵袭力,提示PR异常可能是导致绒毛外滋养细胞侵袭能力发生改变而引起相关疾病的原因之一。Objective： The expression profile of the progesterone - receptor isoforms and progesterone regulation of vascular endothelial growth factor （VEGF） were investigated in human extravillous trophoblast to elucidate the role of progesterone effect on extravillous trophoblast invasion during placental vasculogenesis and angiogenesis and the potential mechanism of maintain pregnancy, Methods： The levels of PRA mRNA, PRB mRNA and VEGF mRNA were determined by RT -PCR and Real -time PCR. Results： The levels of PRB mRNA had no significant difference with distinct progesterone concentration （ P 〉 0. 05 ）. The lack of expression of PRA revealed PRB played dominating roles in extravillous trophoblast rather than PRA. The basal VEGF mRNA expression had no correlation with progesterone concentration, whereas opposed to the level of PRB mRNA, Conclusion ： The role of progesterone might be determined by the levels of PR and an inhibitory role of PRB on expression of VEGF suggest that abnormal level of PR result in dysfunctional regulation of the extravillous trophoblast invasion and induce several complications of pregnancy.

关 键 词：孕激素 孕激素受体 血管内皮生长因子 绒毛外滋养细胞 

分 类 号：R714.147[医药卫生—妇产科学]