康普瑞丁磷酸二钠在体内外的抗肿瘤作用  

Antitumor effects of Combretaftation A-4P in vivo and in vitro

作　　者：牛犇[1] 方素华[2] 包旭[1] 邱果[1] 尹海林[3] 包定元[4] 王红星[2] 徐小平[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川省医学科学院实验动物研究所,四川成都610212 [3]四川大学实验动物中心,四川成都610041 [4]四川大学华西基础医学与法医学院,四川成都610041

出　　处：《华西药学杂志》2007年第6期632-635,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的观察化合物康普瑞丁磷酸二钠（CA-4P）对人早幼粒细胞白血病HL-60细胞的体外抑制作用及对裸小鼠腹腔移植HL-60细胞的体内抗肿瘤作用。方法分别用MTT法、细胞生长曲线及细胞集落形成试验，研究CA-4P对HL-60细胞增殖的抑制作用，台盼蓝染色观察细胞形态变化；HL-60细胞腹腔移植裸小鼠，iv给予CA-4P，qd，5d，观察裸小鼠的腹水形成情况及生存时间，计算生命延长率。结果MTT法提示，0．5～64．0μg·ml^-1 CA-4P对HL-60细胞48h的抑制率为15％-79％，平均IC如约为6．579μg·ml^-1；生长曲线提示，CA-4P对HL-60细胞的抑制作用呈明显时效和量效关系；台盼蓝染色后细胞出现凋亡形态的变化。CA-4P对裸小鼠腹腔移植HL-60细胞的治疗作用呈量效关系，随剂量增大，裸小鼠的生命延长率也增加。结论CA-4P对人早幼粒细胞白血病HL-60细胞的增殖具有抑制作用，对HL-60细胞裸小鼠腹腔移植瘤具治疗作用。OBJECTIVE To investigate the in vivo/in vitro inhibitory effects of compound Combretaftation A -4P（CA -4P） on human leukemia HL -60 cell line and on HL -60 cell inoculated with transplant tumor nude mice. METHODS The inhibitory effect of compound CA -4P on human leukemia cell line HL -60 was assayed by MTT analysis, cell growth curve and clone forming experiments with different concentrations of CA -4P. The change of cell configuration was observed by dyeing. The inhibitory effect of compound CA -4P on nude mice inoculated with HL- 60 cells in vivo was demonstrated by the prolongation of survival times in mice cartied tumor. RESULTS After treated with CA - 4P for 48 hours, MTT assay showed the close range from 0. 5 μg·ml^-1 to 64. 0 μg·ml^-1 had the inhibitory ratio range from 15% to 79%. The average ICso in 48 h was 6. 579 μg·ml^-1. The close - effect relationship was described in the growth curve. The characteristic typical morphology for apoptosis was observed under microscope. The close - effect relationship was also observed in the experimentation of HL - 60 cell inoculated transplant tumor nude mice by CA - 4P, and the prolongation of survival times in mice carried tumor was prolonged along with the rise of CA - 4P concentration. CONCLUSION CA - 4P could inhibit the growth of HL -60 cell in vitro, and its in vivo inhibitory effectin of HL -60 cell inoculated nude mice was distinct.

关 键 词：康普瑞丁磷酸二钠 HL-60细胞 白血病 药理作用 

分 类 号：R965[医药卫生—药理学]