当归拈痛丸的鉴别及黄芩苷的测定  

Identification and content determination of baicalin in Danggui Niantong pill

作　　者：耿红梅[1] 王广庆 段春改 张辉 

机构地区：[1]河北衡水学院应用化学系,河北衡水053000 [2]河北衡水市工人医院,河北衡水053000 [3]河北省药品食品监督管理局,河北石家庄050017 [4]河北衡水市药品检验所,河北衡水053000

出　　处：《华西药学杂志》2007年第6期673-675,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立当归拈痛丸的鉴别和含量测定的方法。方法采用TLC法对处方中的当归、苦参、苍术、葛根、羌活等进行定性鉴别；采用HPLC法测定黄芩中黄芩苷的含量。结果TLC能检出处方中当归、苦参、苍术、葛根、羌活等；HPLC测定时，黄芩苷0．080-0．401μg与峰面积呈良好的线性关系（r=0．9998），平均回收率为98．28％，RSD=1．01％。OBJECTIVE To identify and detemine the content of baicalin in Danggui Niantong pill. METHODS Angelica sinensis, Sophora flavescens, Atractylodes lancea, Pueraria lobata and Notopterygiumfranchetii were identified by TLC. And the content of baicalin in Scutellaria viscidula was determined by HPLC. RESULTS Angelica sinensis, Sophora flavescents, Atractylodes lancea, Pueraria lobata and Notopterygium franchetii could be dentified by TLC. Baicalin content showed a good linear relationship within the range of 0. 080 - 0. 401 μg（ r = 0. 9998 ）. The average recovery was 98.28% with the RSD of 1.01%. CONCLUSION The methods are accurate and reliable, and can be used for the quality control of Danggui Niantong pill.

关 键 词：当归拈痛丸 薄层色谱法 高效液相色谱法 黄芩苷 

分 类 号：R917[医药卫生—药物分析学]