质粒载体pIRES2-FL-IL-3转染对脐血CD34^+干细胞增殖影响的实验研究  

Effect of transfection of plasmid vector pIRES2-FL-IL-3 on proliferation of cord blood stem cells

作　　者：张勇[1] 张林生[1] 郭朝华[2] 张红宾[1] 董世武[2] 

机构地区：[1]第三军医大学西南医院血液科,重庆400038 [2]第三军医大学全军复合伤研究所,重庆400038

出　　处：《重庆医学》2007年第23期2404-2405,2408,共3页Chongqing medicine

基　　金：国家自然科学基金青年基金项目(30200071)。

摘　　要：目的观察真核共表达质粒载体pIRES2-FL-IL-3转染对脐血干细胞增殖水平的影响。方法利用流式细胞检测技术探讨pIRES2-FL-IL-3(共表达组)转染脐血CD34+干细胞后其增殖水平的变化,Western blot技术检测FL和IL-3蛋白表达。另设空白对照组、pIRES2-IL-3组(IL-3组)和pIRES2-FL组(FL组)。结果共表达组细胞增殖指数I、L-3及FL蛋白活性均显著高于对照组I、L-3组和FL组。结论真核共表达质粒载体pIRES2-FL-IL-3转染脐血干细胞后其细胞增殖指数显著提高,与IL-3及FL蛋白活性增加显著相关。Objective To detect the effect of transfection of plasmid vector plRES2-FL-IL-3 on the proliferation of cord blood stem cells. Methods Cord blood stem cells were transfected by plasmid vector plRES2-IL-3, plRES2-FL and plRES2-FL-IL-3. Flow cytometry and Western blot technology were used to detect the proliferation changes of cells and protein activity of FL and IL- 3. Results The protein activity of FL and IL-3 and proliferation index in the plRES2-FL-IL-3 group were higher than that in the control group, plRES2-IL-3 group and plRES2-FL group respectively. Conclusion The proliferation index in plRES2-FL-IL-3 group was higher than that in the other groups, which was related to the co-expression of FL and IL-3.

关 键 词：白介素3 FLT3配体 质粒 细胞周期 胎血 干细胞 

分 类 号：Q78[生物学—分子生物学]