实时荧光定量PCR检测特殊人群中HCV-RNA的研究  

STUDY ON THE REAL-TIME FLUORESCENT QUANTITATIVE PCR IN DETECTION OF HCV-RNA IN SPECIAL PEOPLE

作　　者：刘桂香 吴炳英 田登 

机构地区：[1]青海省疾病控制中心,西宁810000

出　　处：《现代预防医学》2007年第23期4459-4460,共2页Modern Preventive Medicine

摘　　要：[目的]探讨HCV在特殊人群中的流行和干扰素治疗效果对HCV-RNA含量的影响。[方法]收集223份抗-HCV阳性血清并跟踪观察和用荧光定量实时荧光定量PCR法检测HCV-RNA的含量。[结果]在141份抗-HCV阳性血清中,58份实时荧光定量PCR阳性,阳性符合率为41.13%,在86份有输血史患者中39份实时荧光定量PCR阳性,阳性率为45.35%,17份有家族遗传史患者中11份实时荧光定量PCR阳性符合率为64.70%。在38例不明原因感染者中有8份实时荧光定量PCR阳性,阳性率为21.05%(2χ=16.21Р=0.000)。58例实时荧光定量PCR阳性的病人经治疗两年后,39份有输血史患者,转阴24例,转阴率61.53%。11份有家族遗传史患者中6例转阴,转阴率54.54%。在8例不明原因感染者中有5例转阴,转阴率62.24%。[结论]不同的传染途径在RNA的含量和治疗的预后有差异。[Objective]In order to research the prevalence of HCV in the special people and cured effects by interferon.[Methods]223 sera in which anti-HCV were positive were collected and observed,then used the real-time fluorescent quantitative PCR in detecting HCV-RNA.[Results]There were 58 HCV-RNA positive sera in 141 anti-HCV positive sera,the positive rate was 41.13%.There were 39 HCV-RNA positive sera in 86 sera which were from the patients with the history of blood transfusion,the positive rate was 26.82%.There were 11 HCV-RNA positive sera in17 sera which were from the patients with family heredity,the positive rate was 26.82%.There were 8 HCV-RNA positive sera in 38 sera which were from the infected patients with no remarkable reasons,the positive rate was 21.05%.There were remarkable differences among the groups（χ2 = 16.21,Р = 0.000）.58 HCV-RNA positive patients in which 39 patients with the history of blood transfusion were treated with interferon,24 patients＇ sera became negative,the negative rate was 61.53%.The sera of 6 patients with family heredity became negative,the negative rate was 54.54%.The sera of 5 infected patients with no remarkable reasons became negative,the negative rate was 62.24%.[Conclusion]There are differences in the quantity of RNA and cured effects through different spread ways.

关 键 词：实时荧光定量PCR 特殊人群 HCV-RNA 

分 类 号：R512.63[医药卫生—内科学]