三个不同基因型黄瓜再生体系的优化研究  

Studies on Optimizing Cucumber Regeneration System of Three Different Genotypes

作　　者：张卫华[1] 朱妍妍[2] 王志峰[1] 曹齐卫[1] 于玉梅[3] 孙小镭[1] 

机构地区：[1]山东省农业科学院蔬菜研究所,山东济南250100 [2]山东农业大学园艺科学与工程学院,山东泰安271018 [3]湖南农业大学,湖南长沙410128

出　　处：《山东农业科学》2007年第6期5-7,24,共4页Shandong Agricultural Sciences

基　　金：山东省农科院创新基金(2007YCX022)资助;山东省农科院博士基金(2006YBS008)资助

摘　　要：以欧美型、华北型、华南型3个不同基因型的黄瓜为试材,比较了苗龄、激素配比、外植体类型、芽诱导时间的长短以及基因型对再生频率的影响。结果表明,以2天苗龄的黄瓜子叶为外植体,在MS＋6-BA1 mg/L＋ABA1 mg/L＋Ades 20 mg/L的诱导培养基上诱导15天左右,转入MS＋GA30.5 mg/L的芽伸长培养基,3种基因型黄瓜都能获得较高的植株再生率,每个外植体出苗数在1.8以上。从外植体接种到再生苗移栽仅需6-7周。The effects of different age of seedlings, combination of hormones in the medium, explants, time of shoot induction and genotypes on cucumber plantlet regeneration were studied. The results showed that： when cotyledons from two-day seedlings were cultured on shoot induction mediurn, namely MS supplied with 1 mg/L 6-BA, 1 mg/L ABA and 20 mg/L Ades, for about 15 days, then transferred to shoot elongation medium, namely MS supplied with 0.5 mg/L GA3, plantlets could regenerate efficiently from three different genotypic explants. More than 1.8 plantlets could be obtained from every explant. It needed only 6-7 weeks from cotyledon subculturing to plantlet transplanting.

关 键 词：黄瓜 子叶 外植体 植株再生 

分 类 号：S642.243[农业科学—蔬菜学]