毛地黄优良变异植株组织培养及无性系的建立  

Tissue Culture and Establishment of Clonal Propagation of Digitalis purpurea Variant

作　　者：梁佼[1] 程丽娟[1] 陈莹[1] 王黎波[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《山东农业科学》2007年第6期11-13,20,共4页Shandong Agricultural Sciences

基　　金：辽宁师范大学教学改革项目(20050101123)

摘　　要：研究了毛地黄优良变异植株嫩茎愈伤组织的诱导、分化及无性系的建立。结果表明:MS+1mg/L BA+0.6 mg/L 2,4-D是毛地黄嫩茎愈伤组织诱导的理想培养基;MS+0.5 mg/L BA+0.1 mg/LNAA是愈伤组织分化的理想培养基;MS+0.2 mg/L BA+0.2 mg/LIAA是不定芽壮苗培养的理想培养基;1/3 MS+0.3 mg/LIAA是生根培养的理想培养基;炉灰渣、河沙是生根试管苗移栽的理想基质。The experiments for callus induction, differentiation from immature stems, the rooting and transplantation of tube seedlings and establishment of clonal propagation of digitalis purpurea variant was carried out. The results indicated that the ideal medium for induction and differentiation of callus were MS＋1 mg/L BA＋0.6 mg/L 2,4-D and MS＋0. 5 mg/L BA＋0. 1 mg/L NAA respectively. The effective medium for cultivating adventitious buds was MS ＋0.2 mg/L BA ＋0.2 mg/L IAA. The medium of 1/3MS＋0.3 mg/L IAA was suitable for rooting. Cinder and sand were used as the best medium for transplantation.

关 键 词：毛地黄 组织培养 无性系 

分 类 号：S567.210.43[农业科学—中草药栽培]