PCR技术检测孕妇血浆中胎儿DNA相关检测条件的探讨  

Study on the factor affecting the dedection of fetal DNA in maternalplasma by polymerase chain reaction

作　　者：冯霞[1] 胡爱华[2] 刘福民[3] 王秀英[3] 王文[1] 叶月仙[1] 陈红[1] 

机构地区：[1]徐州医学院附属医院中心实验室,江苏徐州221002 [2]中国矿业大学医院妇产科 [3]徐州医学院附属医院妇产科

出　　处：《中国妇幼保健》2007年第34期4925-4927,共3页Maternal and Child Health Care of China

基　　金：江苏省卫生厅重点项目资助:H2001

摘　　要：目的:探讨检测孕妇血浆中胎儿DNA的稳定、灵敏、可靠的方法。方法:应用套式PCR和常规PCR技术,用SRY基因序列作为研究孕妇血浆中胎儿DNA的基因标志。套式PCR检测结果与常规PCR检测结果进行比较分析。结果:套式PCR检测结果优于常规PCR。最早检测出孕妇血浆中胎儿DNA的时间是7-1周。整个孕期均能检测到胎儿游离DNA。检测敏感度为1μg(100000个细胞)女性DNA背景中检测到1个男性DNA。结论:套式PCR技术是检测孕妇血浆中胎儿DNA的稳定、灵敏、可靠的方法。Objective： To find a sensitive and constant methods to detect fetal DNA in maternal plasma. Methods： As a symbol of fetal DNA, SRY gene sequence in maternal plasma was measured by a nest -polymerase chain reaction （PCR） and by a routine PCR. The results by nest PCR were compared to that by routine PCR. Results： The nest PCR was better than routine PCR and that fetal DNA could be detected in maternal plasma during first trimesters （Tlweeks） to at terms. The assay could detect an equivalent of a single male cell in 100000 （1μg） female ceils. Conclusion： Nest PCR is a sensitive and constant methods to detect fetal DNA in maternal plasma.

关 键 词：PCR技术 孕妇 血浆 胎儿DNA 

分 类 号：R714.5[医药卫生—妇产科学]