退变腰椎间盘组织细胞凋亡及相关基因Fas和Bcl-2蛋白的表达  

Apoptosis of Disc Cells and Apoptotic Related Genes Fas and Bcl-2 in Degenerative Lumbar Disc Tissue

作　　者：叶君健[1] 林金贵[2] 

机构地区：[1]福建医科大学附属第一医院骨科,福州350005 [2]福州市第二医院骨科,福州350007

出　　处：《福建医科大学学报》2007年第6期506-508,共3页Journal of Fujian Medical University

基　　金：福建省自然科学基金资助项目(2007J0080)

摘　　要：目的观察髓核组织Fas和Bcl-2蛋白的表达,探讨其在腰椎间盘退变过程可能发挥的作用。方法采用原位DNA片断末端标记(TUNEL)和免疫组织化学方法,检测20例退变腰椎间盘(退变组)及6例正常腰椎间盘(对照组)髓核组织的细胞凋亡状态及凋亡相关基因Fas和Bcl-2的表达。结果退变组及对照组髓核组织细胞凋亡指数(AI)分别为(50.23±13.36)和(28.32±6.67)(P<0.05),Fas蛋白阳性细胞百分比(74.26±10.15)%和(41.72±4.62)%(P<0.01),Bcl-2蛋白阳性细胞百分比(55.37±13.65)%和(27.17±5.17)(P<0.01)。凋亡细胞越多,Fas和Bcl-2的表达越强。结论Fas和Bcl-2蛋白均参与腰椎间盘组织中细胞凋亡的调节,可能在腰椎间盘退变中发挥重要作用。Objective To investigate the relationship between expression of Fas and Bcl-2 protein in apoptotic status and lumbar intervertebral disc degeneration. Methods The apoptotic status and the expression of Fas and Bcl-2 in 20 cases of degenerative lumbar intervertebal disc tissues and 6 of normal lumar intervertebal disc tissues was detected by using TdT-mediated dUTP-biotin nick end labeling （TUNEL） and immunohistochemistry methods. was （50.23±13.36）,（28.32±6.67）（P〈0. 05）. （74.26±10.15）%, （41.72±4.62）% （P〈0. 01）. Results The average apoptotic index（A1） in two groups The expressions rates of Fas protein in two groups was was （55.37±13. 65）%, （27. 17±5. 17） （P〈0. 01）. larger the apoptotic index was. The expressions rates of Bcl-2 protein in two groups The stronger Fas and Bcl-2 protein expressed, the Conclusion Both Fas and Bcl-2 protein probably take part in the apoptosis of nucleus pulposus and the progression of lumbar intervertebal disc degeneration.

关 键 词：椎间盘 腰椎 细胞凋亡 膜糖蛋白类 基因 BCL-2 基因表达 

分 类 号：R681.53[医药卫生—骨科学]