白藜芦醇对肝癌Bel-7404细胞增殖、凋亡及侵袭的影响  

The Effects of Resveratrol on Proliferation,Apoptosis and Invasion of Bel-7404 Hepatocarcinoma Cell Line

作　　者：杜强[1] 陈燕凌[1] 

机构地区：[1]福建医科大学附属协和医院肝胆外科,福州350001

出　　处：《福建医科大学学报》2007年第6期509-513,共5页Journal of Fujian Medical University

摘　　要：目的研究白藜芦醇对肝癌Bel-7404细胞增殖、凋亡及侵袭的影响。方法MTT法和增殖细胞核抗原(PCNA)测定法检测白藜芦醇对Bel-7404细胞增殖的影响;流式细胞术和TdT酶介导的dUTP缺口末端标记法检测白藜芦醇对Bel-7404细胞凋亡的影响;黏附实验、运动实验和侵袭实验检测白藜芦醇对Bel-7404细胞侵袭能力的影响。结果白藜芦醇浓度>25μmol/L可抑制肝癌细胞Bel-7404增殖,并诱导凋亡(P<0.01);浓度>100μmol/L正常肝细胞L02的增殖也受到抑制,并产生凋亡(P<0.01)。25,50μmol/L的白藜芦醇可抑制Bel-7404细胞黏附细胞外基质纤连蛋白(FN)及降解基质的能力(P<0.01),对该细胞的运动能力无明显抑制作用。结论一定浓度的白藜芦醇可抑制肝癌Bel-7404细胞增殖,诱导其凋亡,并可能通过抑制Bel-7404细胞与细胞外基质FN的黏附及对基质的降解来抑制Bel-7404细胞的侵袭能力;在较高浓度下(≥100μmol/L),白藜芦醇对正常肝细胞株也产生抑制增殖和诱导凋亡的毒性作用。Objective To observe the effects of resveratrol on proliferation, apoptosis and inva- sion of Bel- 7404 hepatocarcinoma cell line. Methods MTT and PCNA assay were used to detect the effects of resveratrol on proliferation of Bel-7404. The effect of resveratrol on apoptosis of Bel-7404 was detected by cytometry and TUNEL. Adhesive test, moving test and invasive test were used to observe the effects of resveratrol on invasion ability of Bel- 7404 cell line. Results 25μmol/L resveratrol inhibited proliferation of Bel-7404 and induced apoptosis of it（P〈0.01）. Once the concentration of resveratrol was upon 100μmol/L, proliferation of normal cell L02 was inhibited by resveratrol, and apoptosis was induced by it（P〈0.01）. 25 μmol/L and 50 μmol/L resveratrol could inhibit the Bel-7404 adhesion to fibronectin（FN） and capability of degradating extracellular matrix（P〈0.01）. However, the moving of the cell line was not inhibited. Conclusion Resveratrol inhibit the proliferation and induce the apoptosis of hepatocarcinoma Bel-7404 cell line, and inhibit the invasive ability of Bel-7404 through inhibiting the Bel-7404 adhesion to FN and capability of degradating extracellular matrix. Resveratrol with high concentration（≥100μmol/L） inhibit proliferation of normal cell and induce apoptosis of it.

关 键 词：藜芦生物碱类 癌 肝细胞 肝肿瘤 细胞增殖 细胞凋亡 肿瘤侵润 

分 类 号：R285[医药卫生—中药学] R735.7[医药卫生—中医学]