IL-11在半相合混合骨髓移植造血恢复中作用的研究  

Effect of IL-11 on Haematopoisis in Mice with Half-matched Mixed Bone Tarrow Rransplantation.

作　　者：申红[1] 汪运山[1] 唐天华[2] 任海全[2] 姜国胜[2] 

机构地区：[1]济南市中心医院中心实验室,山东济南250014 [2]山东医学科学院基础所

出　　处：《中国辐射卫生》2007年第4期394-394,396,共2页Chinese Journal of Radiological Health

摘　　要：目的观察白介素-11 IL-11在活化半相合混合骨髓移植小鼠造血恢复中的作用。方法以急性放射病615小鼠模型为受鼠,615×C57BL/6杂交F1代小鼠为半相合供鼠。半相合鼠骨髓和脾细胞中混合一定比例的同基因脾细胞,进行半相合混合骨髓移植。观察移植后小鼠死亡率、白细胞系造血重建、骨髓集落形成、脾结节及病理学改变。结果加用IL-11的半相合混合骨髓移植组(供受体小鼠脾细胞比例2∶1)为外周血白细胞及骨髓集落数量明显高于未加用IL-11组。结论IL-11可以明显促进半相合混合骨髓移植小鼠造血恢复。Objective To observe effect of IL - 11 on hematopoisis in mice with half- matched mixed BMT. Methods Mice 615 with acute radiation disease were used as recipient mice model, with 615×C57BL/6 hybrid F1 mice being half - matched donor mice. Half - matched murine bone marrow and spleen cells mixed with certain percent of syngeneic spleen ceils （2：1） and underwent mixed half - matched BMT to observe their death rate, hematopoietic recovery of WBC lineage, GM - CFU, spleen node count and pathological changes. Results The half - matched mixed 2：1 BMT group with high percentage of syngeneic spleen cells could lessen the extent of GVHD, as manifested by the quick recovery of peripheral blood WBC count and bone marrow GM - CFU formation. Conclusion IL - 11 could promote the recovery of hematopoisis in half - matched mixed BMT mice.

关 键 词：骨髓移植 半相合 GVHD反应 

分 类 号：R818[医药卫生—放射医学]