四川7个地方山羊品种(类群)mtDNA遗传多样性研究  

Study on Mitochondrial DNA Genetic Polymorphism of Seven Indigenous Goat Breeds(Groups) in Sichuan

作　　者：张红平[1] 李利[1] 向德[1] 刘成建[1] 李学伟[1] 

机构地区：[1]四川农业大学动物遗传育种与繁殖学重点实验室,雅安625014

出　　处：《畜牧兽医学报》2007年第12期1300-1305,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家863基金项目(2002AA242051);四川省育种攻关项目(2006-YZGG-16);四川省青年科技基金(2007Q14-051)

摘　　要：测定了四川7个地方山羊品种（类群）43个个体的mtDNA控制区全序列，结果表明：山羊mtDNA控制区全序列长度为1212bp或1213bp，A＋T含量（59．9％）明显高于G＋c含量（40．1％）。共检测到74个变异位点，序列均为中性突变，核苷酸多样度为1．686％，这些差异共定义了27种单倍型，单倍型多样度为0．966，遗传多样性较为丰富，品种（类群）间存在不同程度的遗传分化。7个地方山羊品种（类群）间的遗传距离变异范围为0．0017～0．0306。用MEGA软件的NJ法构建单倍型序列的系统发育无根树，结果表明四川地方山羊品种（类群）有两个母系来源，但是否就对应于角笋骨羊（Capra aegagrus）和捻角山羊（Capra falconeri）两个野生祖先，还有待于进一步研究。Complete sequence of mitochondrial DNA（mtDNA） control region was sequenced in 43 individuals from 7 indigenous goat （Capra hircus） breeds （groups） in Sichuan province. The complete sequence of mtDNA control region was 1 212 or 1 213 base pair, and the content of A＋T （59.9 % ）was higher than that of G＋C（40.1% ）significantly. 74 sites were polymorphic and all of these sequences were neutral mutation. All sequence polymorphic sites defined 27 haplotypes. High nucleotide diversity （1. 868 %） and haplotype diversity （0. 966） were manifested. These results suggested richer genetic diversity within Sichuan indigenous goat populations and genetic divergence among them. The Kimura 2-parameter distance among breeds （groups）varied from 0. 001 7 to 0. 030 6. Phylogenetic unrooted trees constructed with neighbour-joining methods showed two maternal origins in Sichuan indigenous goats. More detailed studies should be continued to indicate whether the two maternal origins are Capra aegagrus and Capra falconeri.

关 键 词：山羊 MTDNA控制区 序列变异性 种群遗传结构 系统发育 

分 类 号：S827.2[农业科学—畜牧学]