猪IGF2基因G3072A位点多态性及其与大白猪初生重和早期生长的关系  

Polymorphism of G3072A in IGF2 Gene and Its Association with Birth Weight and Early Growth Traits in Yorkshire Pigs

作　　者：孙亿[1] 费思清[1] 唐辉[1] 武英[2] 樊新忠[1] 姜运良[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省农业科学院畜牧兽医研究所,济南250100

出　　处：《畜牧兽医学报》2007年第12期1306-1310,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："863"高新技术项目(2006AA10Z1E1)

摘　　要：本研究采用PCR-SSCP技术对猪胰岛素样生长因子2（IGF2）基因G3072A位点在大白、长白和杜洛克猪群体的单核苷酸多态性（SNP）进行了检测，对其与大白猪初生重和早期生长的关系进行了分析。结果表明，在所分析的大白、长白和杜洛克猪群体的511个体中，IGF2基因在该位点均呈现多态性，大白、长白和杜洛克猪群体AA、AB和BB基因型均有分布，且大白和长白猪群体均显著偏离Hardy-Weinberg平衡（P〈0．01），而杜洛克猪群未偏离Hardy-Weinberg平衡（P〉0．05）。测序结果显示，等位基因A和B分别对应于核苷酸A和G，在A等位基因中，有（AG）二核苷酸的插入。关联分析表明，在大白猪群中，AA、AB和BB基因型个体的初生重差异显著（P〈0．05），AA和AB基因型个体的21、28和70日龄体重以及从出生～21日龄、21～28日龄、28～70日龄的日增重差异不显著（P〉0．1）。By use of PCR-SSCP approach, the present study analyzed the single nucleotide polymorphism （SNP） at G3072A site of IGF2 intron 3 in Yorkshire, Landrace and Duroc pigs. The association of the polymorphism with birth weight and early growth traits were as well analyzed in Yorkshire pigs. The results indicated that the genotypes AA,AB and BB were found in Yorkshire, Landrace and Duroc pig populations, and the populations of both Yorkshire and Landrace pigs were in a state of Hardy-Weinberg equilibrium（P〈0.01） ,whereas the Duroc pig population was not（P〈0.05）. Sequencing demonstrated that A and B alleles correspond to nucleotide A and G, respectively. In addition, for A allele, a （AG） dinucleotide was inserted at the 10-11^th position downstream the mutation site. Association analysis indicated that the least square means of birth weight of AA, AB and BB pigs differ significantly （P〈0.05）, whereas body weight at 21, 28 and 70 days and average daily gain of body weight from birth to 21 days,from 21 days to 28 days and from 28 to 70 days of AA and AB individuals were not significantly different in Yorkshire pigs（P〉0.1）.

关 键 词：猪 IGF2基因 G3072A 多态性 初生重 早期生长 

分 类 号：S828.2[农业科学—畜牧学]