L-精氨酸对脂多糖刺激的断奶仔猪肠道损伤的缓解作用  

Alleviation Effect of L-Arginine on the Intestinal Damages of Weanling Piglets Induced by Lipopolysaccharide Challenge

作　　者：黄晶晶[1] 刘玉兰[1] 朱惠玲[1] 侯永清[1] 丁斌鹰[1] 范伟[1] 郭广伦[1] 

机构地区：[1]武汉工业学院饲料科学系动物营养与饲料科学湖北省重点实验室,武汉430023

出　　处：《畜牧兽医学报》2007年第12期1323-1328,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金面上项目(30500362);国家重点基础研究发展计划项目(2004CB117504);湖北省教育厅项目(D200718003);湖北省自然科学基金创新群体项目(2007ABC009)

摘　　要：研究L-精氨酸(Arg)对脂多糖(LPS)刺激的断奶仔猪小肠黏膜蛋白质、DNA、RNA含量、二糖酶活性和血浆D-木糖含量的影响,旨在探讨L-Arg是否可以缓解LPS刺激所导致的肠道损伤。选取24头健康的(21±1)d断奶仔猪,分成4个处理,每个处理6个重复。采用单因子设计:(1)基础日粮(非应激对照组);(2)基础日粮+LPS(应激对照组);(3)基础日粮+LPS+0.5%Arg(0.5%Arg组);(4)基础日粮+LPS+1.0%Arg(1.0%Arg组)。在第16天,给应激对照组、0.5%和1.0%Arg组注射100μg/kg BW的LPS,非应激对照组注射等量的生理盐水。注射LPS 2 h后,按1 mL/kg BW的剂量分别给仔猪灌服D-木糖溶液,注射LPS 3 h后,采血,分离血浆待测;注射LPS后48 h屠宰仔猪,刮取小肠黏膜待测。结果表明:(1)和非应激对照组相比,应激对照组的十二指肠黏膜蛋白质含量,空肠、回肠黏膜DNA含量,空肠黏膜蔗糖酶活性,回肠黏膜乳糖酶、蔗糖酶、麦芽糖酶活性,血浆D-木糖含量均显著降低(P<0.05),这表明LPS刺激导致断奶仔猪肠道损伤;(2)0.5%Arg组十二指肠黏膜蛋白质含量,空肠黏膜DNA含量,回肠黏膜乳糖酶、蔗糖酶、麦芽糖酶活性,血浆D-木糖含量以及1.0%Arg组空肠DNA含量,空肠黏膜蔗糖酶活性,回肠黏膜乳糖酶、蔗糖酶、麦芽糖酶活性,血浆D-木糖含量均高于应激对照组,且与非应激对照组无显著差异(P>0.05)。这表明L-Arg在一定程度上缓解了LPS刺激所造成的肠道损伤。The experiment was conducted to study the effect of L-arginine on the content of protein,DNA, RNA and disaccharidase activities in small intestinal mucosa and the content of D-xylose in plasma of weanling piglets after lipopolysaccharide （LPS） challenge. Pigs were allotted into four treatments （6 replicates/treatment and 1 pig/replicate） including： （1） Nonchallenged control （NONC） ; （2） Challenged control （CHAC）; （3） 0.5% arginine （0.5% Arg）; （4） 1.0% arginine （1.0% Arg）. On d 16 after weanling, pigs in the CHAC, 0.5% and 1.0% Arg groups were injected intraperitoneally with 100 μg/kg BW LPS, whereas pigs in the NONC group were injected with an equivalent amount of sterile saline. D-xylose was orally administrated 2 h at the dose of 1 mL/kg BW after LPS challenge, and blood samples were collected 3 h after LPS challenge. On 48 h after LPS challenge, the pigs were slaughtered to collect intestinal mucosa for analysis. The results showed that： （1） Compared with NONC, the protein content of duodenal mucosa, DNA content of jejunal and ileal mucosa, suctase activities of jejunal mucosa, lactase, suctase, maltase activities of ileal mucosa and D-xylose content of plasma for CHAC were decreased significantly （P〈0.05）, which indicates that LPS induces intestinal damages. （2） Compared with CHAC, the protein content of duodenal mucosa, DNA content of jejunal mucosa, lactase, suctase, maltase activities of ileal mucosa, D-xylose content of plasma for 0.5% Arg group, and DNA content of jejunal mucosa, suctase activities of jejunal mucosa, lactase, suctase, maltase activities of ileal mucosa, D-xylose content of plasma for 1.0% Arg group were numerically higher, and had no significant difference from those of NONC （P〈0.05）, which indicates that L- arginine can alleviate the intestinal damage induced by LPS to some degree.

关 键 词：L-精氨酸 脂多糖 断奶仔猪 肠道损伤 

分 类 号：S828.5[农业科学—畜牧学]