表达H1N1、H3N2猪流感病毒HA基因重组质粒在小鼠的免疫效力  

Immune Efficacy of the Recombinant Plasmids Expressing HA Gene of H1N1 and H3N2 Subtype SIV in Mice

作　　者：胡永浩[1] 包世俊[1] 赵有淑[1] 温峰琴[1] 姚学萍[1] 杨焕良[2] 陈化兰[2] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院哈尔滨兽医研究所,哈尔滨150001

出　　处：《畜牧兽医学报》2007年第12期1410-1413,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业部动物流感重点开放实验室开放课题资助项目

摘　　要：应用已构建的真核表达质粒pCI-H1-HA、pCAGGS-H1-HA、pCI-H3-HA和pCAGGS-H3-HA作为DNA疫苗,利用BALB/c小鼠进行免疫保护试验,通过测定不同免疫期HI抗体滴度、分析攻毒后BALB/c小鼠体重变化及肺脏病毒含量,评价DNA疫苗的免疫效力。结果表明:构建的DNA疫苗均可诱导小鼠产生免疫力;BALB/c小鼠体重变化统计学分析显示,免疫组与对照组差异极显著(P<0.01),pCAGGS表达载体构建的DNA疫苗免疫效果优于pCI表达载体构建的DNA疫苗(P<0.05)。BALB/c mice were inoculated with the recombinant plasmids（pCI-H1-HA,pCAGGS- H1-HA,pCI-H3-HA and pCAGGS-H3-HA） which included the HA gene of H1N1 and H3N2 subtype SIV to screen valuable vaccine candidate. The immune efficacy was evaluated by detecting HI antibody titers,analyzing weight changes of mice and examining virus titers in lungs. The results indicated that the constructed DNA vaccines induced immune responses in BALB/c mice. Meanwhile, by the statistical analysis of the weight changes of BALB/c mice, there were high significant differences between the immunization groups and the control（P〈0.01）, and the DNA vaccines constructed with pCAGGS as the expression vector had better immune efficacy than that of DNA vaccines constructed with pCI（P〈0.05）.

关 键 词：猪流感病毒 HA基因 DNA疫苗 免疫效力 

分 类 号：S855.3[农业科学—临床兽医学] S852.659.5[农业科学—兽医学]