DADS诱导人白血病细胞分化中的代谢变化  

The Change of Cell Metabolism in Human Leukemia Cell Induced by DADS

作　　者：何洁[1] 万炜[2] 廖前进[1] 宋颖[1] 谭晖[1] 苏琦[1] 梁宋平[3] 谢景云[3] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001 [2]南华大学人体解剖学教研室 [3]湖南师范大学生命科学学院

出　　处：《南华大学学报（医学版）》2007年第5期646-649,共4页Journal of Nanhua University(Medical Edition)

基　　金：湖南省卫生厅重点科研基金资助项目(No00068);湖南省教育厅科研基金资助项目(03C415;04B057)

摘　　要：目的研究二烯丙基二硫诱导人髓性白血病HL-60细胞分化过程中蛋白质组的差异表达。方法运用蛋白质组学方法(双向凝胶电泳、质谱分析、生物信息学方法)分析鉴定DADS诱导HL-60细胞分化的差异表达蛋白质。结果初步鉴定的差异表达蛋白质点中二型碳酸酐酶、磷酸丙糖异构酶、醛缩酶A在处理组中均表达下调,其功能与细胞代谢调节有关。结论DADS可通过抑制二型碳酸酐酶表达和抑制糖酵解途径,抑制HL-60细胞增殖,诱导其分化。Objective To study differential proteomic expression in the differentiation of HL - 60 cells induced by diallyl disulfide （DADS）. Methods Differential expression of cellular proteins in HL - 60 cell induced by DADS were analyzed and identified via proteomic approach, two dimensional gel electrophoresis, mass spectroscopy and bioinformatics. Results Among the differential expression protein, the expression of Carbonic anhydrase Ⅱ （ CAⅡ ）, aldolase A, Triosephosphate isomerase was down - regulated in DADS - treated group. These proteins function were associated with cell metabolism. Condusion DADS may inhibit expression of CA Ⅱ and glycolytic pathway which can result in inhibition of of HL - 60 cells proliferation and induction of HL- 60 cells differentiation.

关 键 词：双向电泳 质谱分析 二烯丙基二硫 HL-60细胞 分化 

分 类 号：R979.1[医药卫生—药品] R733.7[医药卫生—药学]