检测肌红蛋白的半定量金免疫层析法的研究  

Development of a semi-quantitative gold immunochromatographic assay for serum myoglobin

作　　者：郑佐娅[1] 曹丹如 陈悦 卢仁泉[1] 刘德琮[3] 朱晴晖[3] 陶义训 

机构地区：[1]上海交通大学医学院医学检验重点实验室,上海200023 [2]上海市临床检验中心,上海200126 [3]上海市普陀区人民医院检验科,上海200060

出　　处：《检验医学》2007年第6期645-647,共3页Laboratory Medicine

摘　　要：目的建立一种半定量检测人血清肌红蛋白的金免疫层析方法,用于急性心肌梗死的快速检测。方法一株抗人肌红蛋白单克隆抗体标记胶体金,另一株抗人肌红蛋白单克隆抗体包被膜作为检测线,兔抗鼠IgG抗体包被膜作为对照线,建立半定量金免疫层析测定方法。结果本法测定结果分为3个半定量判定区段：〈70、70-150和〉150μg/L;与化学发光免疫测定对照检测83份血清标本,符合性良好。结论金免疫层析测定法具有半定量的特点,方法简便、快速、准确,适用于急性心肌梗死的早期检测。Objective To develop a semi-quantitative gold immunochromatographic assay （G1CA） for the detection of serum myoglobin（Mb） from patients with acute myocardial infarction. Methods The GlCA was developed with one anti-human Mb monoclonal antibody labeled with gold sol and another anti-human Mb monoclonal antibody immobilized on the membrane as test line, while a rabbit anti-mouse IgG antibody was immobilized on the membrane as control line. Results The test ranked the results in three regions of Mb ： 〈 70,70 - 150 and 〉 150 Hg/L. The correlation of test results of 83 serum samples was excellent between the GICA and classical chemiluminescence immunoassay. Conclusions The semi-quantitative GICA for Mb is simple, rapid and reliable for clinical application in the early diagnosis of acute myocardial infarction.

关 键 词：肌红蛋白 金免疫层析法 半定量测定 

分 类 号：R446.61[医药卫生—诊断学]