梅毒螺旋体血清学筛查和确诊试验的临床应用  

Clinical application of serologic screening and confirmation tests for Treponema pallidum infection

作　　者：丁双双[1] 沈霞[1] 

机构地区：[1]上海交通大学医学院苏州九龙医院检验科,江苏苏州215021

出　　处：《检验医学》2007年第6期681-683,共3页Laboratory Medicine

摘　　要：目的探讨2种梅毒螺旋体血清学试验同时用于梅毒螺旋体感染的筛检和确诊的重要性和必要性。方法快速血浆反应素环状卡片试验(RPR)及梅毒螺旋体明胶凝集试验(TPPA)。结果对我院6 528名输血前筛查及皮肤科患者的血清样本同时作RPR和TPPA检测,阳性者再进一步检测其滴度。RPR阳性率为1.29%,TPPA阳性率为1.90%;2种方法同时阳性率为1.15%。结论为早期确诊梅毒螺旋体感染并阻断其传播途经,对输血前筛查及疑似梅毒螺旋体感染患者应采用RPR和TPPA2种方法同时进行检测。Objective To study the importance and neeessity of the screening and confirmation of Ttreponema pallidum infection by two serologic tests simultaneously. Methods Serum samples were determined by rapid plasma reagin test （RPR） and Treponema pallidum particle assay （TPPA）. Results Serum samples from 6 528 patients who consulted dermatology department or received blood transfusion were tested simultaneously by RPR and TPPA, and the positive samples were retested for titration. The positive rate was 1.29% by RPR and 1.90% by TPPA, while the positive rate was 1.15% in both tests. Conclusions To early diagnose the infection and block the transmission pathway, the application of two serologic tests simultaneously to detect anti Treponema pallidum antibody is necessary for the screening of blood donor and diagnosis of suspected infection.

关 键 词：梅毒螺旋体 抗体 快速血浆反应素环状卡片试验 梅毒螺旋体明胶凝集试验 

分 类 号：R377[医药卫生—病原生物学]