SO_2衍生物对人BEP2D细胞哮喘基因表达影响  

Effects of SO_2 derivatives on asthma-related gene expressions in human BEP2D cells

作　　者：李瑞金[1] 孟紫强[1] 

机构地区：[1]山西大学环境医学与毒理学研究所环境毒理学研究室,太原030006

出　　处：《中国公共卫生》2007年第12期1457-1459,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(20677035)

摘　　要：目的研究二氧化硫(SO2)衍生物染毒后人支气管上皮细胞(BEP2D)哮喘相关基因黏蛋白5AC(MUC5AC)和白介素-13(IL-13)表达的变化。方法以不同浓度SO2衍生物(0.000 1,0.001,0.01,0.1,1 mmol/L)对BEP2D细胞染毒,采用荧光实时定量RT-PCR、免疫细胞化学和酶联免疫吸附法(ELISA)等方法测定基因的表达。结果不同浓度SO2衍生物处理的BEP2D细胞中MUC5AC和IL-13 mRNA和蛋白表达比对照组明显增加;经0.1 mmol/L SO2衍生物处理后换新鲜培养基再孵育不同的时间,MUC5AC和IL-13 mRNA水平均显著增高,在更换新鲜培养基孵育4 h时达到峰值,24 h时虽然有所降低,但仍比对照组显著增高。结论SO2衍生物能增加EBP2D细胞MUC5AC和IL-13在转录和翻译水平上的表达,提示这可能是SO2加重哮喘疾病的原因之一。Objective To explore the effects of SO2 derivatives on MUC5AC and IL - 13 gene expressions in the human bronchial epithelial cells（BEP2D）. Methods Using BEP2D cells, the mRNA and protein levels of MUC5AC and IL - 13 gene at different concentrations of SO2 derivatives（0.000 1,0. 001,0.1, 1 mmol/L） were measured by real - time RT - PCR technology and immunocytochemistry method, respectively. Results Different concentrations of SO2 derivatives exposure （0. 000 1,0.001,0.01, 0.1, 1 mmol/L） amd different post- exposure time（0.1 mmol/L SO2 derivatives） at 0, 5, 1, 4 and 24 h increased mRNA expressions of MUC5AC and IL - 13 in BEP2D cells compared with the control. Meanwhile, MUC5AC and IL - 13 protein levels in BEP2D cells were increased at different concentrations of SO2 derivatives exposure and different post - exposure time, peaking in 4 h post - exposure and increasing in 24 h post - exposure compared with the control. Conclusion SO2 derivatives exposure could increase the expressions of MUC5AC and IL - 13 genes on the transcription and translation levels in BEP2D cells, suggesting it might be one of the possible reasons that SO2 pollution aggravates asthma disease.

关 键 词：SO2衍生物 哮喘 黏蛋白(mucin MUC)5AC(MUC5AC) 白介素-13(IL-13) 人支气管上皮细胞(BEP2D) 

分 类 号：R994.6[医药卫生—毒理学] R256.12[医药卫生—药学]