牛黄酸和VC对染砷小鼠脑神经损伤保护作用  

Protection of taurine and vitamin C on damage of brain nerve in mice exposed to arsenic

作　　者：朴丰源[1] 杨光[1] 李秋娟[1] 叶建新[1] 孙鲜策[1] 马宁[2] 

机构地区：[1]大连医科大学卫生学教研室,大连116044 [2]日本国立三重大学医学部

出　　处：《中国公共卫生》2007年第12期1467-1469,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(3057158430600488);辽宁省教育厅基金资助(05L113)

摘　　要：目的观察牛黄酸和维生素C(VC)对砷暴露导致的脑组织核酸损伤的保护作用,为砷毒性脑损伤的预防和治疗提供科学依据。方法昆明种小鼠40只,随机分为4组:4 mg/L三氧化二砷染毒组1、50 mg/kg牛黄酸和45mg/kg VC拮抗组以及生理盐水对照组。每周2次,连续染毒60d,取小鼠脑组织固定,用免疫组化方法观察脑组织神经细胞8-硝基鸟嘌呤(8-NO2-G)的表达。结果染砷组小鼠脑组织出现异常病理学改变,脑皮质神经细胞的胞浆中8-NO2-G呈现明显的高表达。而牛黄酸和VC拮抗组小鼠脑组织出现轻度的病理学变化8-NO2-G表达。结论牛黄酸和VC对砷暴露小鼠脑组织RNA损伤具有保护作用。Objective To observe prerention of taurine and vitamin C from RNA damage of cerebral neurons in mice exposed to arsenic. Methods Forty mice were divided into 4 groups, one control and one experimental group receiving As2O3, two antagonistic groups receiving taurine with As2O3 or vitamin C with As2O3, were administered for 60 days. Immunohistochemistry method was used to investigate 8-nitroguanine expression in cerebral neurons of mice. Results The pathological changes and stronger expression of 8-nitroguanine were observed in the brain cortex of mice exposed to arsenic. In the two antagonistic groups, the light pathological changes and exprexssion of 8-nitroguanine were shown in brain tissue of mice. Conclusion Taurine and vitamin C may have the preventive effect on RNA damage of cerebral neurons in mice exposed to arsenic.

关 键 词：三氧化二砷 脑 8-硝基鸟嘌呤 免疫组化 拮抗剂 

分 类 号：R994.4[医药卫生—毒理学]