辽宁省甲1亚型流感病毒HA1基因变异分析  

Analysis on characterization of HA1 gene of influenza virus subtype H1N1 in Liaoning province

作　　者：吴少慧[1] 何雅慧[1] 于伟[1] 张眉眉[1] 崔健秋[1] 刘敏[1] 付荣华[1] 赵晓光[1] 

机构地区：[1]辽宁省疾病预防控制中心传染与感染性性疾病控制所,沈阳110005

出　　处：《中国公共卫生》2007年第12期1522-1523,共2页Chinese Journal of Public Health

基　　金：人事部留学回国人员博士后科研启动基金(LXZZ2005003);教育部留学人员科研基金(2005383;NSFC)

摘　　要：目的了解2000～2006年辽宁省甲1亚型流感病毒HA1基因变异特征。方法利用辽宁省流感监测的咽拭分离株中的甲1亚型流感病毒核酸，采用逆转录-聚合酶链反应（RT—PCR）扩增，测序，并推导出其氨基酸序列，进行基因进化特征分析。结果辽宁省2000～2002年均分离到甲1亚型流感病毒，但消失了近3年后，2005年底又出现，且基因序列碱基发生变异，与2005年以前的病毒株和疫苗株A／New Caledonia／20／1999比较，在其HA1蛋白分子上存在有4个氨基酸位点（187位W〉R，188位Y〉F，209位Q〉K，249位V〉A）发生了替换。基因序列及氨基酸序列同源性均有下降。结论辽宁省2000～2006年甲1亚型流感病毒基因序列发生了明显变异，与甲1亚型疫苗株A／New Caledonia／20／1999同源性有下降趋势，提示其抗原可能发生部分漂移或更换，这对流行季节疫苗选择和流行病学研究都具有重要意义。Objective To characterize HA1 gene of influenza virus subtype H1N1 circulated from 2000 to 2006 in Liaoning local area. Methods Viral RNA was extracted and transcribed into cDNA by reverse transcriptase and amplified by PCR. The product of PCR was purified and sequenced by ABI 3100 avant. The sequence data were analyzed with epidemic records. Results （1）The 21 sequences including those from genbank（except the other earlier 2 sequences） can be roughly divided into two major distinct lineages before and after the year 2000. （2）Four recent Liaoning strain sequences in 2005-2006 have a variation about 3 % compared with the other 5 strains of a few years ago. The nucleic acid and amino acid sequence data of HA1 domain showed that there were four important mutant positions, they were 187 W〉R, 188 Y〉F, 209 Q〉K,249 V〉 A respectively. Conclusion The HA1 domain of HA gene of influenza virus（H1N1） isolated from 2005～2006 showed mutation, and the mutated viruses were becoming the dominant circulating strain in Liaoning local area, and showed amino acid sequence difference compared to A/New Caledonia/20/1999, the vaccine components pronounced by WHO from 1999 to 2006, which suggested that further surveillance should be conducted to monitor the virus mutation in circulation.

关 键 词：流感病毒 甲1亚型 序列分析 

分 类 号：R373.13[医药卫生—病原生物学]