黄瓜中农药残留甲氧虫酰肼的反相高效液相色谱法测定  

Determination of Methoxyfenozide Residues in Cucumber by High Performance Liquid Chromatography

作　　者：赵善仓[1] 郭长英[1] 毛江胜[1] 赵平娟[1] 

机构地区：[1]农业部食品质量监督检验测试中心(济南),济南250100

出　　处：《现代农药》2007年第6期38-39,44,共3页MODERN AGROCHEMICALS

摘　　要：建立了一种反相高效液相色谱法–紫外检测法(HPLC–UV)测定黄瓜中残留甲氧虫酰肼的方法。采用酸化的甲醇高速匀浆提取,正己烷除脂类杂质,二氯甲烷萃取–反萃取去除亲水性杂质,经氟罗里硅土柱层析净化。在Symmetry C18色谱柱上分离,以V(甲醇)∶V(乙腈)∶V(水)=50∶15∶35(pH=7)为流动相,在波长230nm处进行测定。结果表明,甲氧虫酰肼的峰面积与其质量浓度有良好线性关系。其最小检出量为0.01mg/kg(S/N=3),回收率为84.06%～97.93%,标准偏差小于5.92。该法简便快速、灵敏准确。A high performance liquid chromatographic using UV（HPLC-UV） detection was developed for determination of methoxyfenozide residues in cucumber. Samples were extracted by blending with acid methanol/water. The extract was purified first by n-hexane,to remove fatty impurities then extracted with dichloromethane to discard water-soluble impurities ,and finally cleaning by florisil column chromatography. The separation was performed on Symmetry shieldTM RP18 （5 μm, 4.6 mm×250 mm） column. Methanol-acetonitrile-H20 （V/V/V, 50： 15： 35, pH=7） was used as the mobile phase with a flow rate of 1.0 mL/min. The detection wavelength was 230 nm. The good linearity was obtained for methoxyfenozide. The detection limited was 0.01 mg/kg. The average recoveries were between 84.06% and 97.93%, and standard deviations was less than 5.92. The methods is effective, fast and accurate.

关 键 词：高效液相色谱法 甲氧虫酰肼 黄瓜 农药残留 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]