绿僵菌素的分离制备及其对蛴螬的毒力  

Purification of destruxins produced by Metarhizium anisopliae and bioassay of their insecticidal activities against grubs

作　　者：胡琼波[1] 任顺祥[1] 刘树艳[1] 

机构地区：[1]华南农业大学资源环境学院

出　　处：《昆虫学报》2007年第5期461-466,共6页Acta Entomologica Sinica

基　　金：国家"十五"攻关项目(2004BA509B0604);广东省自然科学基金项目(010312)

摘　　要：以金龟子绿僵菌金龟子变种Metarhizium anisopliae var.anisopliae菌株MaQ10发酵液为材料,采用萃取、浓缩、制备色谱及重结晶技术,分离纯化出5种绿僵菌素的晶体,经与标准样品以及参考文献相对照,此5种晶体与绿僵菌素A、A2、B、C和E相吻合。进而,采用浸渍法测定了绿僵菌素A和B对大等鳃金龟Exolontha serrulata（Gyllenhall）和卵圆齿爪鳃金龟Holotrichia ovata Chang1龄幼虫的触杀毒力。结果表明：绿僵菌素A和B对大等鳃金龟的触杀活性,在处理后96~120h内最高,LC50分别为78.1571mg/L和88.7562mg/L;处理浓度为300mg/L时,LT50分别为13.4159h和10.5331h。绿僵菌素A和B对卵圆齿爪鳃金龟的触杀活性,在处理后96h时最高,LC50分别为66.5308mg/L和79.4309mg/L;处理浓度为300mg/L时,LT50分别是13.6399h和9.9451h。Five crystal destruxins were purified from the fermentation broth of Metarhizium anisopliae var. anisopliae strain MaQ10 via preparative HPLC and re-crystalization, which were classified to destruxin A, B, C, E and A2, respectively. Contact toxicities of destruxins A and B against the grubs Exolontha serrulata （Gyllenhall） and Holotrichia ovata Chang were bioassayed by immersing the insects in aqueous dilutions. The results indicated that at 96 and 120 h after treatment, the LCsos of destruxins A and B were estimated as 78. 1571 mg/L and 88.7562 mg/L towards E. serrulata and 66.5308 mg/L and 79.4309 mg/L towards H. ovata, respectively. At the concentration of 300 rag/L, the LTs0s of destruxins A and B were 13.4159 h and 10.5331 h towards E. serrulata, and 13.6399 h and 9.9451 h towards H. ovata, respectively.

关 键 词：绿僵菌素 分离制备 毒力 大等鳃金龟 卵圆齿爪鳃金龟 

分 类 号：Q965[生物学—昆虫学]