中国黄粉蝶亚科六属间基于COⅡ和EF-1α基因部分序列的系统发育关系(鳞翅目:粉蝶科)  

Phylogenetic relationship of six genera of the Coliadinae (Lepidoptera:Pieridae) in China based on partial sequences of EF-1α and COⅡ genes

作　　者：刘晓燕[1] 吴孝兵[1] 诸立新[1] 

机构地区：[1]安徽师范大学生命科学学院

出　　处：《昆虫学报》2007年第6期604-609,共6页Acta Entomologica Sinica

基　　金：安徽省优秀青年基金(04043049);安徽省学术与技术带头人专项基金;安徽省高校生物环境与生态安全重点实验室基金

摘　　要：为了探讨中国黄粉蝶亚科属间的系统发育关系,我们对其中6属9种的细胞色素氧化酶Ⅱ(COⅡ)的部分序列和延伸因子基因(EF-1α)部分序列进行了分析。分别采用最大简约法(maximum parsimony,MP)、最大似然法(maximum likelihood,ML)和贝叶斯推论法(bayesian inference,BI)构建黄粉蝶亚科分子系统树。结果表明:在测得的COⅡ基因的648bp序列和EF-1α基因的504bp序列中,有261个变异位点,151个简约信息位点,黄粉蝶亚科内各属COⅡ基因A+T含量(77.3%)均明显偏高。系统发育分析显示黄粉蝶属为亚科中较为原始的类群,分化较早,豆粉蝶属和迁粉蝶属亲缘关系较近,但钩粉蝶属与豆粉蝶属、迁粉蝶属之间的亲缘关系还不能确定。本研究结果和传统的基于形态学的黄粉蝶亚科的分类体系有所不同,最显著的分歧是本研究支持内群中分化最早的属应为黄粉蝶属,而不是豆粉蝶属和迁粉蝶属。The phylogenetic relationships among 9 species in 6 genera of butterflies belonging to the subfamily Coliadinae were explored based on the partial sequences of EF-1α （about 648 bp） and COⅡ （about 504 bp） genes. There were 261 variation sites and 151 parsimony-informative sites, and a 77.3% A ＋ T bias in 1 152 bp nucleotides of the combined sequence of the EF-1α and COⅡ in the species examined. Molecular phylogenetic trees were constructed by using maximum parsimony （MP）, maximum likelihood （ML） and bayesian inference （BI） methods. The results suggested that Eurema was a primordial genus of Colidinae, which was differentiated earlier than other genera. According to the phylogenetic tree, Colias and Catopsilia were located in a sister group and had a close relationship. Whereas, Gonepteryx had an uncertain relationship with the sister group of Colias and Catopsilia. The inferred molecular phylogeny in the study did not support the traditional classification of Coliadinae based on morphology. Especially, this study supported the viewpoint that the earliest genus differentiated from ingroup was Eurema, but not the sister group of Colias and Catopsilia .

关 键 词：鳞翅目 黄粉蝶亚科 EF-1α基因 COⅡ基因 系统发育 

分 类 号：Q969[生物学—昆虫学]