家蚕蛹期雌雄生殖腺蛋白质双向电泳比较分析  

Comparison of proteins from the pupal gonads of silkworms (Bombyx mori) of both sexes by two dimensional electrophoresis

作　　者：毛立明[1] 林健荣[1] 赵峰[1] 钟杨生[1] 王叶元[1] 孔庆明[1] 徐秋云[1] 

机构地区：[1]华南农业大学动物科学学院蚕丝科学系,广州510642

出　　处：《昆虫学报》2007年第6期628-633,共6页Acta Entomologica Sinica

基　　金：广东省科技计划项目(2005B40101012);广东省农业科技计划项目[粤财农(2004)295号]

摘　　要：分析家蚕Bombyxmori雌雄生殖腺细胞蛋白质,有利于发现性别分化相关的功能蛋白质,探讨生殖腺发育相关基因的表达调控机理。本研究利用蛋白质双向凝胶电泳和图像分析技术,分析家蚕蛹期第2天的雌雄生殖腺细胞蛋白质。结果表明:在雄蚕生殖腺蛋白质电泳图谱中共检测到435个蛋白斑点,其中特异性蛋白斑点73个,占总蛋白斑点数的16.8%;雌蚕生殖腺的电泳图谱中有417个蛋白斑点,其中特异性蛋白斑点55个,占总蛋白斑点数的13.2%。雌雄能匹配的蛋白斑点有362对,匹配率达85.0%。The comparison of protein patterns between male and female gonads will not only contribute to the finding of functional proteins related to sexual traits, but also help exploring the regulating mechanism of related genes during the development of gonad. In this study, using two dimensional electrophoresis （2-DE） and gel image analysis system, we compared the proteins in the male and female gonads of silkworm at the 2nd day of the pupal stage. A total of 435 protein spots were detected in the 2-DE pattern of male gonad, of which 73 were the specific protein spots, occupying 16.8% of the total. There were 417 protein spots in the 2-DE pattern of female gonad, of which 55 were specific ones, occupying 13.2% of the total. Comparison showed that 362 pairs were matched in the 2-DE patterns of both male and female, with the match percentage as high as 85.0%.

关 键 词：家蚕 生殖腺 蛋白质 双向电泳 图谱分析 

分 类 号：S882.6[农业科学—特种经济动物饲养]