柱前衍生化-毛细管气相色谱法分析井冈霉素A含量  

Analysis of Jinggangmycin A by Capmary Gas Chromatography with Precolumn Derivatization

作　　者：许鹏军[1] 江树人[1] 张红艳[1] 赵玉珍[1] 杨维华[1] 

机构地区：[1]中国农业大学理学院,北京100094

出　　处：《农药》2007年第12期832-833,836,共3页Agrochemicals

摘　　要：建立了以N，O-双（三甲基硅烷基）乙酰胺（BSA）作衍生化试剂，对井冈霉素A进行气相色谱-FID分析的方法，采用HP-5 MS毛细管色谱柱，进样口温度240℃，检测器温度280℃，采用外标法对井冈霉素原药定量分析，衍生物在4．4-71mg／L范围内呈良好的线性关系（r^2为0．9983）。仪器最低检出质量浓度为4．4mg／L，该方法变异系数为0．29％，回收率为98．46％-101．32％。A method for the determination of jinggangmycin A derivative by gas chromatography （GC） with flame ionization detector （FID） and HP-5 MS capillary column was proposed. Jinggangmycin A was derivatized with N,O- bis（trimethylsilyl）acetamide. Inlet and detector temperatures are 240 ℃ and 280 ℃, respectively. It was quantified with external standard method. Derivative showed good linearity in the range of 4.4 to 71 mg/L （r^2=0.9983）. The limit of detection of jinggangmycin A derivative was 4.4 mg/L. The recoveries range from 98.46 to 101.32%, with relative standard deviation 0.29%.

关 键 词：井冈霉素 气相色谱 衍生化 农用抗生素 

分 类 号：TQ450[化学工程—农药化工]