桔小实蝇线粒体基因组全序列及其分析  

Sequencing and analysis of the complete mitochondrial genome of the Oriental fruit fly,Bactrocera dorsalis (Hendel) (Diptera:Tephritidae)

作　　者：徐浪[1] 余道坚[1] 张润杰[2] 李建光[3] 康林[1] 陈志粦[1] 焦懿[1] 

机构地区：[1]深圳出入境检验检疫局动植中心,广东深圳518001 [2]中山大学昆虫学研究所 [3]北京出入境检验检疫局,北京100026

出　　处：《昆虫学报》2007年第8期755-761,共7页Acta Entomologica Sinica

基　　金：国家奥运科技项目(2004BA904B06);国家"973"项目(2002CB111405);国家自然科学基金项目(30471162)

摘　　要：桔小实蝇Bactrocera dorsalis线粒体基因组全序列对研究实蝇分子系统进化具有重要意义。本研究通过DNA测序和克隆技术,对桔小实蝇mtDNA全序列进行了测定和分析。结果表明:桔小实蝇线粒体基因组全长15915bp(GenBank序列号:DQ845759)。基因组碱基组成为39.3%A,16.2%C,10.2%G,34.3%T,由13个蛋白编码基因、22个tRNA基因、2个rRNA基因以及一个非编码的控制区域(A+T-rich区)组成。7个蛋白编码基因和13个tRNA基因从J链编码,其余6个蛋白编码基因和9个tRNA基因从N链编码。位于J链上的蛋白编码基因具有近似的A、T含量,而位于N链上的蛋白编码基因的A的含量明显高于T的含量。以mtDNACOⅠ基因为例,比较了桔小实蝇与其他14种实蝇的亲缘关系,结果显示其与同亚属(果实蝇亚属Bactrocera)内的其他近缘种相互间的同源性很高。The complete mitochondrial genome of the Oriental fruit fly, Bactrocera dorsalis is important for general molecular and evolutionary studies in Tephritidae. Via sequencing and cloning the target DNA sequences, a complete mitochondrial genome sequence of B. dorsalis from the Chinese population was determined and analyzed. The results indicated that the complete mitochondrial genome of B. dorsalis is a circular molecule of 15 915 nucleotides （GenBank accession no.： DQ845759）. Its overall composition is 39.3%A, 16.2%C, 10.2%G, and 34.3%T, and the gene content includes 22 tRNAs, 2 rRNAs and a long non-coding region （A＋T-rich region）. Seven protein genes and 13 tRNA genes are transcribed from J strand, and the other 6 protein genes and 9 tRNA genes are transcribed genes from N strand. Protein coding genes on J strand show a fairly similar A% and T%, whereas those encoded on N strand have a higher proportion of A than T. The similarity analysis of mtDNA COⅠ genes between B. dorsalis and the other 14 fruit flies showed that there was a high similarity among B. dorsalis and the other species belonging to the same subgenus Bactrocera.

关 键 词：双翅目 实蝇科 桔小实蝇 线粒体基因组 

分 类 号：Q966[生物学—昆虫学]