亚低温对缺氧缺血新生鼠脑源性神经营养因子mRNA表达的影响  

Effets of Hypothermia on BDNFmPNA of Neonatal Rats with Hypoxic-ischemic Pain Injury

作　　者：殷宪敏[1] 杨晓雯[1] 朱桂玉[1] 李静[1] 杨明锋[1] 刘钢[1] 

机构地区：[1]泰山医学院附属医院,山东泰安271000

出　　处：《泰山医学院学报》2007年第3期161-164,共4页Journal of Taishan Medical College

基　　金：山东省卫生厅科研基金资助项目(编号:2003HW143)

摘　　要：目的探讨亚低温对缺氧缺血脑损伤(HIBD)新生大鼠脑源性神经营养因子(BDNF))mRNA表达的影响。方法新生7日龄Wistar大鼠120只,随机分为①假手术组(n=6);②31℃亚低温干预组(n=36);③34℃亚低温干预组(n=36);④非亚低温干预组(对照组)(n=36);⑤正常对照组(n=6)。用原位杂交技术测定BD-NF mRNA。用Tunel染色和免疫组化技术检测神经元凋亡数。结果HIBD缺血再灌注后脑组织有BDNF mRNA表达的增加;亚低温组HIBD脑组织BDNFmRNA表达与未干预组比较差异有统计学意义。亚低温组脑组织海马、皮层区细胞凋亡数与未干预组比较差异有统计学意义。结论亚低温可通过增加HIBD后脑组织海马、皮层BDN-FmRNA的表达水平,抑制细胞凋亡而发挥脑保护作用。Objective：To study the effect of hypothermia on BDNFmRNA of neonatal rats with hypoxic-ischemic brain injury.Methods：Acording to the random serial number,7 days old Wistar rats weighing 13~16g were divided into five groups： normal control group（n=6）,sham operated group（n=6）,HIBD group（n=36）,31 ℃ hypothermia group（n=36） and 34 ℃ hypothermia group（n=36）.BDNFmRNA in the brain was measured using situ hybridization.Neuron apoptosis was tested by Tunel and HE kit.Results： 1.The increased level of BDNFmRNA was found in the dentate gyrus and hippocamapal CA1 after HIBD.2.The level of BDNFmRNA in hypothermia group was higher than that in HIBD group without hypothermia.3.The positive cells of apoptosis in hypothermia group dropped obviously compared with HIBD group.Conclusion： The hypothermia can increase the level of BDNFmRNA and decrease the apoptosis cells in oder to protect the brain injury.

关 键 词：亚低温 脑缺氧脑缺血 神经营养因子 脑源性 细胞凋亡 大鼠 新生 

分 类 号：R722.1[医药卫生—儿科]