一株石油烃降解菌的筛选及性能评价  

Screening and performance evaluation of one petroleum hydrocarbon degrading bacterium

作　　者：周金葵 王大威[2] 廖明清[3] 刘永建[2] 

机构地区：[1]大庆职业技术学院,黑龙江大庆163712 [2]大庆石油学院,黑龙江大庆163318 [3]大庆油田有限责任公司测试技术服务分公司,黑龙江大庆163412

出　　处：《大庆石油地质与开发》2007年第6期119-123,共5页Petroleum Geology & Oilfield Development in Daqing

基　　金：国家级重大基础研究前期研究专项(2005CCA06200);黑龙江省自然科学基金项目(E200523)联合资助

摘　　要：从大庆油田分离到一株可降解石油烃的微生物菌株,经鉴定为洋葱伯克霍尔德氏菌(Burkhold-eria cepacia)。菌株经过了室内生化特性、产生活性物质乳化原油的能力等详细的评价。结果显示,原油在该菌种作用后轻质组分增加,原油的族组成发生了变化,粘度下降,改善了原油流动性质。岩心物理模型驱油实验证明,微生物驱比水驱提高采收率8.2%左右,并具有较好的重复性。One bacterium, Burkholderia cepacia, which can degrade crude oil, has screened from Daqing oilfield. The biochemical properties and capability to create active material for oil emulsification have been evaluated in lab. The results suggest that after the effect of this kind of bacterium, the components of oil have changed with the increase of light component, decrease of viscosity and improvement of oil flow properties. According to core simulation, the recovery ratio of MEOR can be 8. 2%.higher than that of waterflooding, and MEOR can be used for multi times.

关 键 词：微生物采油 洋葱伯克霍尔德氏菌 石油烃降解 

分 类 号：TE357[石油与天然气工程—油气田开发工程]