人颗粒溶素的生物学活性研究  

The biological activity of human granulysin

作　　者：张黎[1] 伊正君[1] 杨春[1] 何永林[1] 朱道银[1] 

机构地区：[1]重庆医科大学免疫学教研室,400016

出　　处：《国际生物制品学杂志》2007年第6期244-247,共4页International Journal of Biologicals

基　　金：国家自然科学基金(30400375)

摘　　要：目的全面研究 Mr 9000人颗粒溶素(GLS)的生物学活性,为其作为生物治疗剂应用奠定基础。方法将含 Mr9000GLS 编码基因的 pZM03质粒转染肝癌细胞株 SMMC-7721,通过 MTT 实验、流式细胞仪、Hoechst 染色法检测 GLS 的致肿瘤细胞凋亡活性;将 pZM03转染已感染结核分枝杆菌毒力株 H37Rv 的 RAW264.7细胞,96 h 后裂解细胞作细菌培养及菌落计数,检测 GLS 的细胞内直接杀菌活性。结果将 pZM03质粒转染 SMMC-7721细胞后,从多个角度检测到了 GLS 的致肿瘤细胞凋亡活性;转染荷菌的 RAW264.7细胞96 h 后,菌落计数实验证明 GLS 具有一定的细胞内直接杀菌活性。结论 pZM03质粒编码的 Mrg000GLS 具有天然 GLS 的杀瘤活性和一定的细胞内直接杀菌活性。Objective To investigate the biological activity of Mr 9000 human granulysin for the application as a biological therapeutic agent. Methods The eukaryotie plasmid pZM03 was transfeeted into the SMMC-7721 （a cell line of human hepatoma） and the cell apoptosis was detected by MTT assay, flow cytometry and Hoeehst staining. The bactericidal activity of GLS was examined by counting the colonies of H37Rv survived in maerophages RAW 264.7 transfected with pZM03 at 96 h. Results The capability of GLS for inducing apoptosis in tumor cells was confirmed by MTT assay, flow eytometry and Hoeehst staining. The colony counts showed that GLS had some bactericidal activity for Mycobacterium tuberculosis. Conclusions The GLS expressed by maerophages transfeeted with the eukaryotie plasmid pZM03 does have the ability of inducing apoptosis in tumor cells and killing intraeellular Mycobacterium tuberculosis.

关 键 词：颗粒溶素 肿瘤 分枝杆菌 结核 

分 类 号：R392[医药卫生—免疫学]