偶氮氯膦Ⅲ与人血清白蛋白相互作用的电化学研究  

Electrochemical Studies on the Interaction of Chlorophosphonazo Ⅲ with Human Serum Albumin

作　　者：赵娜[1] 牛学良[1] 王艳[1] 孙伟[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042

出　　处：《化学研究》2007年第4期79-82,共4页Chemical Research

基　　金：山东省教育厅实验技术改革项目;青岛科技大学教学研究立项项目

摘　　要：偶氮氯膦Ⅲ是一种具有电化学活性的染料,在pH3.5的Britton-Robinson缓冲溶液中,它可以与人血清白蛋白发生相互作用形成一种生物超分子复合物,使溶液中游离的染料浓度降低.以线性扫描二阶导数极谱法对偶氮氯膦Ⅲ-人血清白蛋白的相互作用体系进行了详细的研究,复合物的形成使偶氮氯膦Ⅲ在-0.124V(vs.SCE)的还原峰电流降低,考察了结合反应的最佳条件和测定条件,求解了结合常数和结合比.The interaction of chlorophosphonazoⅢ （ CPA Ⅲ） with human serum albumin （HSA） was investigated with linear sweep voltammetry on the mercury working electrode. In pH 3.5 Britton-Robinson （B-R） buffer solution, CPAⅢ had a sensitive reductive peak at -0. 124 V （vs. SCE）. With the addition of HSA into the CPA Ⅲ solution, the reductive peak current of CPA Ⅲ decreased apparently. The conditions of interaction and the electrochemical detection were carefully investigated. The interaction mechanism was discussed with the aggregation of HSA-CPAⅢ supramolecular complex and the stoichiometry of the supramolecular complex was calculated with the binding number as 3 and the binding constant as 1.48× 10^13.

关 键 词：人血清白蛋白 偶氮氯膦Ⅲ 电化学 超分子复合物 结合反应 

分 类 号：O657.1[理学—分析化学]