关木通对大鼠肾功能和组织病理学的影响  

Effects of caulis aristolochiac manshuriensis on renal function and histopathology in rats

作　　者：魏春利[1] 西月[2] 李才[2] 苗春生[2] 曲锐[3] 李立国[4] 

机构地区：[1]吉林公安高等专科学校刑事技术教研室,吉林长春130117 [2]吉林大学药学院病理毒理学教研室,吉林长春130021 [3]吉林大学第一医院心血管内科,吉林长春130021 [4]吉林省公安厅刑事科学鉴定中心,吉林长春130061

出　　处：《吉林大学学报（医学版）》2007年第6期960-962,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30570855)

摘　　要：目的:观察大鼠关木通灌胃后肾功能及肾脏组织病理学改变。方法:雄性Wistar大鼠随机分为对照组和模型组。模型组在给予关木通3、7和15 d时,采血并留取24 h尿液,分别检测血肌酐、尿素氮、24 h尿蛋白及尿视黄醇结合蛋白(RBP);在上述时间点观察肾脏组织病理学改变,并与对照组对比。结果:①与对照组比较,模型组大鼠给予关木通后不同时间血肌酐、尿素氮及24 h尿蛋白排泄率无明显升高;与对照组比较,尿RBP含量给药后3 d即显著增加(P<0.01),并随着给药时间的延长持续升高。②模型组大鼠给药后3 dHE染色可见局部肾小管上皮细胞变性、坏死和崩解,并随给药时间的延长逐渐加重。结论:关木通致大鼠肾损伤的主要病理改变为肾小管上皮细胞变性、坏死。Objective To investigative the changes in renal function and histopathological in caulis aristolochiac manshuriensis （CAM） -induced injury in rats. Methods Male Wistar rats were randomly divided into control group （n=10） and administration group （n=30）. After CAM were administered by gavage to rats for 3, 7, 15 d,the blood and 24 h-urine samples were collected, 24 h-urinary protein excretion, urinary retinol-binding protein （RBP）, serum creatinine （Scr） and blood urea nitrgen （BUN） were examined. The pathological changes in kidney were observed at various time and compared with control group. Results ① At various time after administration of CAM, the Scr, BUN and 24 h-urinary protein excretion levels were not increased compared with control group. Compared with control group, after administration of CAM for 3 d, urinary RBP content was significantly increased （ P〈0.01）, and the values increased with the elongation of time. ②The degeneration and necrosis in tubules were major pathological changes in rats after administration of CAM for 3 d, and the pathological changes were aggravation as treatment time increased. Oonclusion The degeneration and necrosis in tubules are major pathological changes in rats after administration of CAM.

关 键 词：关木通 肾/病理学 

分 类 号：R285[医药卫生—中药学]