氧化苦参碱对缺血再灌注致心律失常的影响及其机制  

Effects of oxymatrine on arrhythmia induced by ischemia-reperfusion and mechanisms

作　　者：韩丹丹[1] 张文杰[2] 刘洁[3] 陈燕萍[4] 马兴元[4] 陈霞[1] 

机构地区：[1]吉林大学基础医学院药理学教研室,吉林长春130021 [2]吉林大学基础医学院生理学教研室,吉林长春130021 [3]吉林大学基础医学院机能科学实验中心,吉林长春130021 [4]吉林大学化学学院,吉林长春130012

出　　处：《吉林大学学报（医学版）》2007年第6期1047-1049,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省中医药管理局资助课题(2004077)

摘　　要：目的:观察氧化苦参碱(OMT)对大鼠缺血再灌注所致心律失常的影响,并初步探讨其作用机制。方法:结扎大鼠左冠状动脉前降支5 min后再灌注10 min制备缺血再灌注性心律失常模型,分为模型组及OMT15、30和60 mg.kg-1组,观察再灌注后10 min内大鼠室性早搏(VP)出现时间、室性心动过速(VT)持续时间及所有类型心律失常的持续时间。将家兔离体心室乳头肌分为对照组、不同浓度OMT组及OMT+TEA(K+通道阻断剂)组,观察其动作电位的变化。结果:与模型组比较,OMT 15、30和60 mg.kg-1组VP出现时间延迟、VT持续时间缩短(P<0.05,P<0.01),OMT 30和60 mg.kg-1组心律失常持续时间缩短(P<0.01),并且呈明显的剂量依赖性;与对照组比较,OMT 0.1、1.0和10.0 mmol.L-1组家兔离体心室乳头肌动作电位幅值(APA)减小,动作电位10%、50%及90%复极化时程(APD10、APD50及APD90)明显缩短(P<0.05,P<0.01),且TEA可减弱其作用。结论:OMT可对抗缺血再灌注所致大鼠的心律失常,其机制可能与缩短动作电位时程有关。Objective To observe the effects of oxymatrine （OMT） on arrhythmia induced by ischemia-reperfusion and explore its mechanism. Methods The left arterial descending coronary artery （LAD） was ligated for 5 min followed by 10 min reperfusion in order to induce ischemia-reperfusion arrhythmia in rats. Rats were randomly divided into; model group, OMT groups with different doses （15, 30, and 60 mg · kg^- l）. The emergency time of ventricular premature （VP）, and the duration of ventricular tachycardia （VT） and arrhythmia induced by ischemiareperfusion were observed. The isolated rabbit papillary muscles were divided into control group, OMT groups with different doses and OMT-F TEA group. The action potientials in various groups were observed. Results Compared with model group, OMT delayed the emergency time and decreased the duration of arrhythmia induced by ischemia-reperfusion in dose-dependent manner in rats （P〈0.05, P〈0. 01）. Compared with control group, the 10%, 50% and 90% repolarization levels were shortened by OMT in isolated rabbit papillary muscles （P〈0. 05, P〈 0. 01）. The effects were blocked by TEA. Conclusion OMT may antagonize the arrhythmia induced by isehemia-reperfusion in rats, its mechanism may be related with shortening action potential duration （APD）.

关 键 词：氧化苦参碱 缺血再灌注 心律失常 动作电位 离体乳头肌 

分 类 号：R285.5[医药卫生—中药学]