电离辐射对人HepG2肝癌细胞caspase-3蛋白表达的影响  

EFFECTS OF IONIZING RADIATION ON CASPASE-3 PROTEIN EXPRESSION IN HUMAN HEPG2 CELLS

作　　者：龚明玉[1] 张力[2] 王凯华[2] 

机构地区：[1]承德医学院生物化学教研室,河北承德067000 [2]承德医学院附属医院

出　　处：《承德医学院学报》2007年第4期350-352,共3页Journal of Chengde Medical University

摘　　要：目的:观察电离辐射对人He-pG2肝癌细胞Caspase-3蛋白表达的影响。方法:采用6MV-X射线照射人HepG2肝癌细胞,Western blotting蛋白印迹法检测细胞凋亡蛋白Caspase-3的表达。结果:6MV-X照射后48小时,2Gy、4Gy、6Gy剂量组HepG2细胞Caspase-3蛋白的表达明显高于假照射对照组(0Gy)(P<0.05,P<0.01)。结论:电离辐射可诱导HepG2细胞Caspase-3蛋白表达增高。Objective：To investigate the effects of ionizing radiation on Caspase-3 protein expression in human HepG2 cells.Methods：HepG2 cells were irradiated with 0Gy,2Gy,4Gy,6Gy,8Gy and 10Gy 6MV X-ray respectively and western blotting was used to detect the expression of Caspase-3 protein in cells 48h after irradiation.Results：The Caspase-3 protein expression in HepG2 cells increased significantly after irradiated by 2Gy,4Gy and 6Gy X-ray compared with sham-irradiation （P 〈 0.05, P 〈 0.01）.Conclusions.Caspase-3 protein expression in HepG2 cells could be induced increasing by X-ray.

关 键 词：电离辐射 CASPASE-3 人肝癌细胞株HEPG2 

分 类 号：R815[医药卫生—放射医学]