PPARɑ激动剂抑制脂多糖诱导的单核细胞组织因子表达和促凝血活性  

PPARα Agonist Inhibits LPS-Induced Tissue Factor Expression and Procoagulant Activity in Monocytes

作　　者：饶绘[1] 魏文宁[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022

出　　处：《血栓与止血学》2007年第6期252-254,260,共4页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的研究过氧化物酶体增殖物激活受体ɑ(PPARɑ)激动剂(非诺贝特)对细菌脂多糖(LPS)诱导的单核细胞株THP-1细胞的组织因子(TF)表达和促凝血活性(PCA)的影响。方法用非诺贝特预处理THP-1细胞,分别用流式细胞术(FCM)和改良组织因子凝血时间法(TiFaCT)检测LPS刺激下THP-1细胞的TF蛋白表达水平和TF-PCA。结果非诺贝特抑制LPS诱导下THP-1细胞的TF蛋白表达上调,并以浓度依赖的方式抑制LPS诱导下的TF-PCA增加(F=62.79,P<0.05)。50μmol/L和100μmol/L浓度的非诺贝特分别使TF-PCA下降到LPS组的29%和25%。结论PPARɑ激动剂抑制LPS诱导的单核细胞TF蛋白表达和TF-PCA,这可能是PPARɑ激动剂减少动脉粥样硬化血栓形成并发症的机制之一,并在血栓性疾病的防治中具有潜在的临床价值。Objective To investigated the effects of peroxisome proliferators-activated receptor-α （PPARα） agonist （fenofibrate） on the expression and procoagulant activity （PCA） of tissue factor （TF） in- duced by Lipopolysaccharide （LPS） in THP-1 cells. Methods THP-1 cells were pretreated with fenofibrate for defnite time. LPS- induced TF protein levels and TF-PCA were measured by flow cytometry and modified Tissue Factor Clotting Time （TiFaCT） respectively. Results Fenofibrate inhibited TF expression, de- creased TF-PCA in a concentration-dependent manner in LPS-stimulated THP-1 cells （F = 62.79, P 〈0. 05 ）. Compared with the TF-PCA of LPS-stimulated THP-1 cells, pretreated with 50 μmol/L or 100 μmol/ L fenofibrate on THP- 1 cells, TF-PCA were decreased to 29% and 25 % respectively. Conclusions These data indicate that PPARα agonist inhibits LPS- induced TF expression and TF- PCA, which may be one of the mechanisms of anti-atherosclerotic thrombosis effects of PPARα agonist, and may be involved the precaution and therapy of thrombosis.

关 键 词：组织因子 过氧化物酶体增殖物激活受体Α 非诺贝特 血栓形成 

分 类 号：R972.6[医药卫生—药品]