利用转基因技术获得抗病陆地棉的初步研究  

Preliminary Study on Obtaining Disease-resistant Upland Cotton by Transgenic Technology

作　　者：王冬梅[1] 李建平[1] 张艳红[1] 周小云[1] 黄乐平[1] 

机构地区：[1]新疆农科院核生所,乌鲁木齐830091

出　　处：《新疆农业科学》2007年第B11期108-110,共3页Xinjiang Agricultural Sciences

基　　金：新疆农作物细胞工程重点实验室课题

摘　　要：由于棉花的枯、黄萎病日益严重,选育抗病品种成为育种的首要任务。为提高棉花的抗病性,利用花粉管通道法将从拟南芥中分离克隆的抗病基因snc1转入中棉所35号中,通过除草剂田间筛选和目的基因的PCR检测,获得转snc1基因的棉花,同时确定除草剂的筛选浓度为0.025%。也为棉花的抗病机理研究做有益探索。When cotton wilt is becoming increasingly cerious it is very important to breed disease - resistance cotton. In order to crease the resistance of cotton, by means of pollen- tube pathway, sncl （supressor of nprl - 1,constitution 1 ） cloned from Arabidopsis was transferred into ＂Zhongmiansuo 35＂. By selecting of bastar and PCR, transgenic cottons were obtained, and selecting the concentration of bastar was 0. 025 %. It is useful exploration to study mechanism of disease - resistance cotton.

关 键 词：抗病基因sncl 花粉管通道法 陆地棉 

分 类 号：S562.035.2[农业科学—作物学]