微板法检测淡色库蚊非特异性酯酶实验研究  

Microplate Assay for the Determination of Non-specific Esterase in Culex pipiens pallens

作　　者：王新国[1,2] 赵玉强[1,2] 甄天民 

机构地区：[1]山东省寄生虫病防治研究所 [2]济宁,272133

出　　处：《中国媒介生物学及控制杂志》1997年第3期172-174,共3页Chinese Journal of Vector Biology and Control

基　　金：山东省卫生厅资助

摘　　要：目的：探讨蚊虫抗药性敏感测试方法。方法：采用微板法，对室内常规饲养的敏感品系和室内选育的抗DDVP、抗残杀威品系淡色库蚊非特异性酯酶的活性进行了测定。结果：敏感品系蚊虫OD值≥0．9的占17．16％（35／204条）；＜0．9的占82．84％（169／204条）；抗DDVP品系OD值≥0．9的占83.33％（200／240条）；＜0.9的占16.67％（40／240条）；抗残杀威品系≥0.9的占74.89％（176／235条），＜0．9的占25．11％（59／235条）。结论：敏感品系与抗性品系淡色库蚊的平均OD值存在较大差异。Microplate assay was employed for the determination of nonspecific esterase level in individual susceptible,anti-DDVP and anti-propoxur resistant Culex pipiens pallens. Among susceptible mosquitoes detected, 17. 16% was of high esterase activity with OD value more than 0. 9. Among anti-DDVP and antipropoxur resistant mosquitoes, the OD value more than 0. 9 was found in 83. 33 % and 74. 89 % respectively.This indicated that there existed obvious difference in the level of nonspecific esterase between susceptible and resistant mosquitoes.

关 键 词：微板法 淡色库蚊 非特异性酯酶 

分 类 号：R384.1[医药卫生—医学寄生虫学]