杀虫药剂抗性家蝇品系乙酰胆碱酯酶基因的特征分析  

Characterization of Acetylcholinesterase Gene from Insecticide resistant Housefile ( Musca domestica )

作　　者：黄瑶[1] 乔传令[1] 

机构地区：[1]中国科学院动物研究所虫鼠害国家重点实验室

出　　处：《生物工程学报》1997年第3期258-263,共6页Chinese Journal of Biotechnology

基　　金：国家自然科学基金

摘　　要：乙酰胆碱酯酶（ＡＣｈＥ）是有机磷和氨基甲酸酯类杀虫药剂的作用靶标，这两大类杀虫药剂的广泛应用导致了昆虫对抗性的选择。靶标的修饰是某些昆虫产生抗性的分子机理，这种抗性是和ＡＣｈＥ的变更型相关的，这些变更型的酶显示出对杀虫药剂的不敏感性。利用ＲＴＰＣＲ和Ｓｔｒｅｐｔａｖｉｄｉｎ偶联磁珠技术从两种抗性家蝇（Ｍｕｓｃａｄｏｍｅｓｔｉｃａ）品系Ｄ３和Ｋａｓｈ中分别分离了ＡＣｈＥ基因并测定了其核苷酸顺序，ｃＤＮＡ的可读框长２０８２ｂｐ，由此推导出了ＡＣｈＥ的氨基酸顺序，通过与敏感家蝇品系Ｃｏｏｐｅｒ的比较，发现了一些核苷酸顺序差异和４个氨基酸点突变，其中３个替代可能与杀虫药剂不敏感性有关。Acetylcholinesterase (AChE) is the target site for the organophosphates and carbamates in insects.Widespread use of these two classes of insecticides has led to the selection of resistance.Target modification was regarded as a molecular mechanism in some resistance specics.The altered AChEs with reduced sensitive to inhibition are related to this resistance.AChE genes from two insecticide resistant housefily ( Musca domeslica ) strains D3 and Kash were isolated and sequenced using RT-PCR and streptavidin linked magnetic bead techniques.The cDNAs have a 2082 bp open reading frame from which the complete amino acid sequence of AChE has been deduced .Some differences of nucleotide sequence and four point mutations of amino acid were found compared with that of a susceptible strain Cooper.Three substitutions are likely to confer insecticide insensitivity,which seems that D3 and Kash belong to CII2 pattern of resistance.

关 键 词：家蝇 乙酰胆碱酯酶 基因 杀虫药剂 抗性 

分 类 号：Q969.453.8[生物学—昆虫学] R384.2[医药卫生—医学寄生虫学]