巨噬细胞修饰低密度脂蛋白对细胞受体活性的影响及前列腺素E_2的作用  

Influence of Cell-modified Low Density Lipoprotein on Cellullar Receptors and Effect of Prostaglandin E_2

作　　者：刘术娟[1] 邱近明[1] 孙保存[1] 赵秀兰[1] 

机构地区：[1]天津医科大学病理学教研室,天津300070

出　　处：《中国动脉硬化杂志》1997年第2期121-125,共5页Chinese Journal of Arteriosclerosis

摘　　要：为研究低密度脂蛋白经细胞修饰后对细胞受体的影响及前列腺素E2的作用，利用免疫细胞化学和生物亲和素-酶联免疫吸附法分别检测小鼠腹腔巨噬细胞清道夫受体、小鼠皮肤纤维母细胞低密度脂蛋白受体的结合活性，结果发现修饰组的巨噬细胞胞浆及胞膜有强阳性黄褐色颗粒，而药物组仅中度着色；修饰组低密度脂蛋白与其受体结合量（54±13μg／g细胞蛋白）较对照组（144±μg／g细胞蛋白）显著下降（P＜0．01）；药物组受体结合量（100±11μg／g细胞蛋白）较修饰组明显提高（P＜0．05）。表明细胞修饰低密度脂蛋白能够刺激清道夫受体活性，抑制低密度脂蛋白受体活性；前列腺素E2（20mg／L）可对抗细胞修饰低密度脂蛋白对此二种受体的作用。Aim To investigate the influence of cell-modifiedlow density lipoprotein(cm-LDL) on scavenger recep-tors and low density lipoprotein receptors (LDLR ),meanwhile the effect of prostaglandin E2 (PGE2) wasstudied.Methods The receptor activity was detected bymeans of immunocytochemistry and bitin avidin-en-zyme linked immunosorbent assay(BA-ELISA), usingcultured murine peritoneal macrophages and murineskin fibroblasts as models.Results 1. The scavenger receptor activity onmacrophages increased in cm-LDL group, showingheavy brown granules in the membrane and cytoplasmof enlarged cells, while in PGE2 group, most cellswere mildly stained and in control group stained weak-ly. 2. The binding amount of LDL to LDLR on fi-broblasts showed that it markedly decreased in cm-LDL group (54±13 μg/g cell protein ) compared withwhich in control group (144± 8μg/g cell protein), P<0. 01 ; and significantly enhanced in PGE, group (100± 11μg/g cell protein), versus which in cm-LDLgroup, P<0. 05..Conclusion The findings suggested that cm-LDLstimulated scavenger receptor and inhibited LDLR,PGE2 (20 mg/L) markedly inhibited scavenger recep-tor activity and protected the LDLR against the injuryof oxidized LDL.

关 键 词：低密度脂蛋白 受体 动脉粥样硬化 前列腺素E2 

分 类 号：R543.502[医药卫生—心血管疾病] R977.6[医药卫生—内科学]