家兔腹主动脉球囊导管损伤后血管平滑肌细胞凋亡和一氧化氮合成酶基因表达  

Apoptosis of Vascular Smooth Muscle Cells and Inducible Nitric Oxide Synthetase Gene Expression in the Rabbit Aorta Injury Model

作　　者：陈颜芳[1] 林曙光[1] 余细勇[1] 伍淑英[1] 夏学峰[2] 

机构地区：[1]广东省心血管病研究所,广州510100 [2]广州医学院神经科学研究所

出　　处：《中国动脉硬化杂志》1997年第2期130-133,共4页Chinese Journal of Arteriosclerosis

摘　　要：采用脱氧核苷酸转移酶介导的缺口末端标记法，观察兔腹主动脉经球囊导管损伤后0、8、24h，3天和5天时血管中膜平行肌细胞和外膜细胞凋亡的变化．提取血管组织RNA，采用反转录一多聚酶链反应测定一氧化氮合成酶基因表达。结果表明，血管损伤后8h可见中膜平滑肌细胞凋亡增加，24h达高峰，3天和5天仍维持在较高水平，外膜细胞凋亡无明显改变。而诱导型一氧化氮合成酶基因表达亦于8h明显增加，在24h达最高，与细胞凋亡存在平行关系。提示中膜平滑肌细胞凋亡是球囊导管损伤血管的一个早期反应，诱导型一氧化氮合成酸可能参与了此过程。Aim This study was to investigate the aspect ofsmooth muscle cell (SMC) apoptosis and inducible ni-tric oxide synthetase (iNOS) gene expression in theearly periods of rabbit aorta injury model.Metkods The endothelium of rabbit abdominalaorta was injuried by a ballon angioplasty catheter,the presence of apoptotic SMC was demonstrated byterminal deoxynucleotidyl transferase-mediated dUTPnick end labeling (TUNEL). The expression of iNOSmRNA was eveluated by reverse transcription-poly-merase chain reaction (RT-PCR) methods.Results TUNEL analysis showed that the mediaSMC underwent apoptosis after injury. The index ofapoptotic SMC was 6% at 0 hour. It was significantlyincreased t0 15% at 8 hours, and reach a maxium(50%) at 24 hours, and maintained a high level at 3days (32% ) and 5 days (20% ). No significant incre-ment of apoptotic cells was observed in adventitia inthe early periods of artery injury. The expression of i-NOS gene mRNA in artery was significantly increasedafter injury, and was parallel with cell apoptotic in-dex.Couclusion Vascular media SMC of rabbit abdomi-nal aorta underwent apoptotic cell death in the earlyperiods of injury caused by ballon catheter. The in-duction of apoptosis coincided with iNOS mRNA ex-pression.

关 键 词：血管损伤 平滑肌细胞 细胞凋亡 一氧化氮合成酶 

分 类 号：R543.02[医药卫生—心血管疾病]