人卵磷脂胆固醇酰基转移酶基因在肌源细胞中的表达  

The Expression of Human Lecithin Cholesterol Acyltrausferase in Myogenic Cells

作　　者：范乐明[1] 朱宇[1] 王南[1] 蔡海江[1] 

机构地区：[1]南京医科大学动脉粥样硬化研究中心,南京210029

出　　处：《中国动脉硬化杂志》1997年第2期148-149,共2页Chinese Journal of Arteriosclerosis

摘　　要：为探索人卵磷脂-胆固醇酰基转移酶在骨骼肌细胞表达的可能性，构建了一个由人巨细胞病毒早期增强子和促进子驱动的卵磷脂-胆固醇酰基转移酶cDNA表达质粒。以阳离子介导其转染小鼠肌源性细胞株C2，获得成功表达，并在细胞培养液内测得有生物活性的卵磷脂胆固醇酰基转移酶。提示有可能通过将卵磷脂胆固醇酰基转移酶基因转移至骨骼肌细胞的方法来提高人体内卵磷脂胆固醇酰基转移酶水平。To study the possibility of expressionof human lecithin cholesterol acyltransferase (LCAT)in skeletal muscles. Constraction of a plasmid vectorcontaining the human LCAT cDNA: transfer the vec-tor into myogenic cell line C2, and detection of LCATactivity in the culture medium. A expression vector ofhuman LCAT drived by CMV E/P, PCMV-LCAT wasconstructed, which successfully expressed in C2 cells.The LCAT activity was detected in the culture medi-um. It seems possible to chance plasma LCAT level bytransfer of LCAT gene into skeletal muscle.

关 键 词：卵磷脂胆固醇 酰基转移酶 肌细胞 动脉粥样硬化 

分 类 号：R543.505[医药卫生—心血管疾病]