柞蚕雄蛾提取液改善W256荷瘤大鼠放疗后免疫功能的实验研究  

Effect of an extract from the male zooid of Antheraea pernyi on reversing radiation-induced immunosuppression in rats bearing W256 carcinoma

作　　者：张维东[1] 张月英[1] 王朝霞[1] 贾青[1] 王兆朋[1] 于金明[2] 

机构地区：[1]山东省医学科学院基础医学研究所山东省现代医用药物与技术重点实验室,济南250062 [2]山东省肿瘤防治研究院,济南250117

出　　处：《山东大学学报（医学版）》2007年第11期1085-1087,1091,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(30472260);山东省自然科学基金(Y2006C92)

摘　　要：目的观察柞蚕雄蛾提取液对荷W256癌肉瘤大鼠放疗后免疫功能的影响。方法60只Wistar大鼠随机分为正常对照组、模型组、单纯放疗组和实验组(放疗与柞蚕蛾提取液联合治疗组),每组15只。建立W256荷瘤大鼠模型,分离脾脏和胸腺,并计算脏器指数;采用流式细胞术(FCM)及酶联免疫吸附试验(ELISA)分别检测外周血T淋巴细胞亚群变化和血清中细胞因子INF-γ和IL-4含量。结果与放疗组相比较,柞蚕雄蛾提取液可以升高放疗后荷瘤大鼠脾脏和胸腺指数,增加放疗后荷瘤大鼠外周血中CD4+T细胞比例与CD4+T/CD8+T值(P<0.05);实验组荷瘤大鼠血清中细胞因子INF-γ含量明显升高,而IL-4含量显著降低(P<0.05)。结论柞蚕雄蛾提取液可以逆转荷瘤大鼠放疗后免疫功能抑制状态,改变荷瘤机体免疫功能,可作为恶性肿瘤传统放疗中的辅助用药。Objective To study the effects of an extract from the male zooid on reversing the radiation-induced im- munosuppression of rats bearing W256 carcinoma. Methods Tumor models were established by subcutaneously injecting W256 tumor cells into rots. 60 Wistar rots were randomly divided into 4 groups. The thymus index and the spleen index were used as parameters to evaluate the effect of an extract from the male zooid on non-specific and specific immune function of rats with tumor. The presence of Interferongamma （INF-γ） and interleukin-4 （IL-4） in the peripheral blood serum were screened by enzyme-linked immunosorbent assay （ELISA） to investigate cell-mediated immune responses and T subsets were determined by flow cytometry（FCM）. Results The extract from the male zooid significantly raised the thymus index and the spleen index of rots with tumors. The number of CD4^＋ T and the CD4^＋ T/CD8^＋ T ratio of W256-bearing rots treated with the extract from the male zooid significantly increased compared with the radiotherapy group （ P 〈 0.05）. Also, the level of Interferon-gamma （INF-γ） was up-regulated and interleukin -4（IL-4） was down-regulated. Conclusions The extract from the male zooid of Antheraea pemyi has effect of reversing the radiation-induced and couldbe regarded as a main assistant agent combined with traditional radiotherapy for treatment of malignant tumors.

关 键 词：柞蚕 TH1/TH2漂移 T淋巴细胞亚群 大鼠 Wistar 

分 类 号：R979.1[医药卫生—药品] R-332[医药卫生—药学]