利用生物反应器大量生产人参不定根  

Mass Producing of Adventitious Roots of Ginseng by Using Bioreactor

作　　者：朴炫春[1] 廉美兰[1] 王守明[1] 

机构地区：[1]延边大学农学院园艺系,吉林龙井133400

出　　处：《林业科技》2007年第6期54-56,共3页Forestry Science & Technology

基　　金：国家自然科学基金资助项目(30560094);教育部科学技术重点项目(205035)

摘　　要：在5 L气球型生物反应器中,采用完全浸没式培养方式,人参不定根鲜重和干重明显优于间歇浸没式培养;在培养开始时一次性注入含50 g/L蔗糖的培养基,可促进人参不定根鲜重和干重的增加;接种时,将不定根切成1 cm长、接种量为20 g时接种较适宜;增殖培养周期以30天为宜。In 5L balloon -type of bioreactor, the adventitious root of ginseng was growth well in immersion culture. Its fresh and dry weight in immersion culture obviously exceed than in ebb -flood culture. The adventitious root growth was promoted by the treatment which the sugar concentration of 50 g/L. Otherwise, It was more suitable that the adventitious roots were cut by 1 cm of size as explants. Besides, inoculation density of 20 g was better. Large numbers of adventitious root growth was found after 30 days of culture period, and it could provide most adventitious root for enhancing ginsenosides at next stage.

关 键 词：人参 不定裉 生物反应器 

分 类 号：S522.01[农业科学—作物学]