己烯雌酚摄入对大鼠生精细胞凋亡及Bax和Bcl-2蛋白表达的影响  

Effects of exposure of male rats to diethylstilbestrol on the apoptosis of spermatogenic cells and the expressions of Bax and Bcl-2

作　　者：甘为民[1] 陈琦[1] 李和程[1] 王子明[1] 程伟[1] 种铁[1] 陈海文[1] 石涛[1] 邱曙东[2] 葛玲[2] 王新阳[3] 

机构地区：[1]西安交通大学医学院第二附属医院泌尿外科,西安710004 [2]西安交通大学医学院人体解剖与组织胚胎学系生殖医学研究中心 [3]西安交通大学医学院第一附属医院泌尿外科

出　　处：《中国男科学杂志》2007年第11期11-14,共4页Chinese Journal of Andrology

基　　金：陕西省科学技术研究发展计划项目[2005K15-G2(5)]资助

摘　　要：目的研究青春期己烯雌酚(diethylstilbestrol,DES)摄入对SD(Sprague-Dawley)大鼠性成熟后睾丸生精细胞凋亡的影响,并初步探讨其机制。方法30只35d龄雄性SD大鼠,随机分为DES 0.01、0.1、1.0、10.0μg/kg·d^(-1)4个实验组和1个对照组(编码为BDa、BDb、BDc、BDd和BC组,每组n=6)。于青春期[出生后第36天(postnatal day 36,PND 36)至49d(PND 49)],实验组每日皮下注射相应剂量的DES,对照组仅注射溶媒。于大鼠性成熟后(PND 64)处死各组大鼠切取双侧睾丸,采用TUNEL法检测大鼠睾丸生精细胞凋亡,用免疫组化方法检测凋亡相关蛋白Bcl-2和Bax在生精细胞中的表达。结果与对照组相比,BDa组大鼠性成熟后生精细胞凋亡无明显变化,BDb、BDc和BDd 3组生精细胞凋亡增加,且随DES摄入剂量增加而有增加趋势。BC、BDa组生精细胞Bax相对弱表达而Bcl-2强表达,伴随DES摄入剂量增加,Bax表达逐渐增强而Bcl-2表达逐渐减弱,BDd组Bax强表达而Bcl-2弱表达。结论青春期较大剂量DES摄入可使大鼠性成熟后睾丸生精细胞凋亡增加,且随DES摄入剂量增加而有加强趋势。凋亡相关蛋白Bax和Bcl-2参与青春期DES摄入所致的生精细胞凋亡过程。Objective To study the effect of Pubertal exposure of male SD （Sprague-Dawley） rats to diethylstilbestrol （DES） on the apoptosis of spermatogenic cells after sexual maturation and its mechanism. Methods Thirty 35-day-old male SD rats were randomly divided into 4 experimental groups, DES 0.01, 0.1, 1.0 and 10.0 μg/kg·d^-1 and 1 control group. The experimental groups were injected （s.c.） with different doses of DES （dissolved in corn oil）during puberty [from postnatal day （PND） 36 to PND 49] and the control group with cehicle only. The apoptosis and related proteins Bcl-2 and Bax expressions of testicular spennatogenic cells were studied with TUNEL and immunohistochemistry after the rats sexual maturation （at PND 64）. Results Compared with the control group, the apoptosis of testicular spermatogenic cells in the DES 0.01 μg/kg group had no difference, but significantly increased in the DES 0.1, 1.0 and 10.0 μg/kg groups and the apoptosis increased with the increase of DES dose. In the control and DES 0.01 μg/kg groups, Bax protein expressed weakly but Bcl-2 protein strongly in spermatogenic cells. With the increase of DES exposure, Bax protein expression in spermatogenic cells increased but Bcl-2 protein expression decreased. Conclusion Pubertal exposure of SD rats to inappropriate dose of DES can make the apoptosis of spermatogenic cells increase after sexual maturation. Bax and Bcl-2 proteins participate in the apoptotic course caused by pubertal DES exposure.

关 键 词：己烯雌酚 生精细胞 细胞 凋亡 大鼠 青春期 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]