围生期母鼠双酚A暴露对断乳期雄性子代大鼠脑组织中雌激素受体mRNA表达的影响  

Effects of Perinatal Exposure to Bisphenol A on Expression of ER mRNA in Cerebral Tissue of Weaned Male Offspring of Rats

作　　者：龙冬梅[1] 程薇波[2] 张浩[2] 吴德生[2] 

机构地区：[1]成都军区疾病预防控制中心卫生监督科,四川成都610021 [2]四川大学华西公共卫生学院,四川成都610041

出　　处：《环境与健康杂志》2007年第5期281-283,共3页Journal of Environment and Health

基　　金：国家自然科学基金重点资助项目(30030120)

摘　　要：目的研究围生期母鼠双酚A(BPA)暴露对断乳期雄性子代大鼠脑中雌激素受体(ERα和ERβ)mRNA表达的影响,探索BPA影响脑发育的机制。方法雌雄SD大鼠分笼饲养,按雌∶雄为2∶1合笼交配。将妊娠大鼠随机分为高、中、低剂量组[BPA的染毒浓度分别为100、20、2mg/(kg·d)]和1个对照组(食用色拉油)进行灌胃染毒。在出生后第21天,取雄性仔鼠脑组织,提取总RNA。于260、280nm处测定RNA的浓度及纯度,采用琼脂糖凝胶电泳分析RNA完整性。以核糖体蛋白L19(RPL19)作为内参照,采用RT-PCR方法检测脑组织中ERα和ERβ mRNA水平的表达。结果高、中、低剂量组雄性仔鼠脑组织中ERα、ERβ与RPL19的积分吸光度值之比均高于对照组,差异均有统计学意义(P<0.05)。结论围生期暴露于BPA可以增加断乳期雄性子代大鼠脑组织中ERα和ERβ mRNA的表达,提示受体表达的改变可能是BPA影响脑发育的机制之一。Objective To study the effects of perinatal exposure to bisphenol A （BPA） on the expression of estrogen receptor α and β mRNA in the brain of F1 male offsprings. Methods Pregnant SD rats were given BPA at 2, 20, 100 mg/kg bw per day respectively from eleventh day of gestation throughout the whole lactation by gastric gavage until their pups were weaned on postnatal day 21, F1 male pups from each group were killed on postnatal day 21 respectively, the brain was removed for detecting the expression of estrogen receptor α and β mRNA by RT-PCR. Results BPA treatment caused a up-regulation of ERoα mRNA and ERβ mRNA relative expression in the brain, especially in the middle-dose and low-dose groups it was so obvious. Conclusion Perinatal exposure to BPA can cause a change of ERoα mRNA and ERβ mRNA expression in the brain of the male offspring,which may be a part of the mechanism of BPA indrieed brain development damage.

关 键 词：环境内分泌干扰物 雌激素受体 脑 双酚A 

分 类 号：R994.6[医药卫生—毒理学]