孕期和哺乳期母鼠饮水砷暴露对子代大鼠脑组织神经递质代谢酶的影响  

Effects of Inorganic Arsenic Exposure on GS and AChE Activities in Brain of Offspring Rats

作　　者：孙文娟[1] 王凤芝[1] 席淑华[2] 孙贵范[2] 金亚平[2] 

机构地区：[1]沈阳医学院预防医学系环境卫生学教研室,辽宁沈阳110034 [2]中国医科大学公共卫生学院,辽宁沈阳110034

出　　处：《环境与健康杂志》2007年第5期342-343,共2页Journal of Environment and Health

摘　　要：目的了解砷对神经递质代谢酶的影响,探讨砷对学习、记忆能力影响的机制。方法选取清洁级Wistar大鼠,按雌∶雄为2∶1合笼。将64只孕鼠随机分为3个暴露组(分别为10、50、100mg/L剂量组)和1个对照组(蒸馏水)。暴露组于妊娠第6天(胎鼠器官分化开始)开始用NaAsO2溶液进行饮水染毒。仔鼠出生后,母鼠继续染毒。仔鼠出生后28d断乳,染毒至出生后第42天。在仔鼠出生后第0、28、42天,测量其大脑皮质和海马中谷氨酰胺合酶(GS)、乙酰胆碱酯酶(AChE)活力。结果出生后第0、28、42天,各暴露组子鼠脑皮质和海马中GS活力与对照组比较,经方差分析,差异均无统计学意义(P>0.05)。出生后第0天,各组仔鼠的脑组织中AChE活力比较,经方差分析,差异无统计学意义(P>0.05)。出生后第28、42天,100mg/L组子鼠脑海马中AChE活力高于对照组,差异均有统计学意义(P<0.05);且AChE活力均随染毒剂量的增加而增强,相关系数分别为0.408和0.374,呈统计学正相关(P<0.05),有明显的剂量-效应关系;其他各组仔鼠脑皮质AChE活力比较,经方差分析,差异均无统计学意义(P>0.05)。结论砷暴露能够引起发育中的仔鼠脑组织AChE活力改变,可能会导致相应的中枢神经系统的一些改变。Objective To study the effects of inorganic arsenic on the activities of GS and AChE in the central nervous system of the offspring rats. Methods Wistar rats were exposed to arsenitc through drinking water at doses of 10, 50 and 100 mg/L respectively from gestation day 6 until F1 pups 42 days old. The activities of ACHE, GS in rat brain regions such as cortex and hippocampus were separately determined in F1 pups 0, 28, 42 days old. Results On the postnatal day 0, there were not any significant changes in AChE and GS activities in arsenic group rats compared with the control rats. An increase of AChE in 100 mg/L arsenic group rats hippocampus was showed on the postnatal day 28.These changes also appeared on the postnatal day 42. Conclusion Consecutive arsenic exposure from embryo to postnatal may induce the activity changes of GS, AChE in the pups brain, which may cause results in the related neurotransmitter concentration changes.

关 键 词：砷中毒 大鼠 酶类 

分 类 号：R994.6[医药卫生—毒理学]