多器官功能障碍综合征患儿外周血单个核细胞核因子-κB的活性及相关炎性因子的变化  

Change of nuclear factor Kappa B and correlated cytokines in children with multiple organ dysfunction syndrome

作　　者：韦茹[1] 曾华松[1] 赵明奇[1] 陶丽[1] 杨镒宇[1] 曾萍[1] 张剑辉[1] 曾其毅[1] 

机构地区：[1]广州市儿童医院,广东广州510120

出　　处：《中国实用儿科杂志》2007年第12期901-904,共4页Chinese Journal of Practical Pediatrics

基　　金：广东省科技厅资助项目(编号:2003B30503);广州市科技攻关重大项目(编号:2003Z2-E0181);广州市卫生局重点项目(编号:2005-Zdi-08)

摘　　要：目的探讨外周血单个核细胞核因子-κB(NF-κB)活性及相关炎性因子白介素-1β(IL-1β)、IL-6、IL-8、肿瘤坏死因子-α(TNF-α)、IL-4和IL-10在小儿多器官功能障碍综合征(MODS)中的作用及临床意义。方法2004-10—2005-06,于广州市儿童医院采用酶联免疫吸附法(ELISA)检测28例MODS患儿第1、3、7天3个时点外周血单个核细胞NF-κB活性,同时采用Luminex TM-100液相分析平台测定血清IL-1β、IL-6、IL-8、TNF-α、IL-4和IL-10质量浓度,并设立对照组20例。结果MODS患儿各时点单个核细胞NF-κB活性、血清IL-6、IL-10明显高于对照组(P<0.05),在存活组呈降低趋势而死亡组维持在高水平(P<0.05),TNF-α显著下降;NF-κB活性与TNF-α负相关(r=0.333,P=0.002),与IL-6正相关(r=0.292,P=0.007)。结论MODS患儿外周血单个核细胞NF-κB活性、血清IL-6、IL-10水平的持续升高预示预后不良。Objective To observe the change of nuclear factor Kappa B （NF-κB） and correlated cytokines（ IL-1β,IL- 6, IL-8,TNF-α, IL-4 and IL-10） in children with multiple organ dysfunction syndrome （ MODS）, and to evaluate their potent role in the process of MODS. Methods The activation of NF-κB was measured by enzyme-linked immunosorbent assay （ELISA） in 28 children with MODS at day 1,3 and 7 ,who were divided into a group of the recovered（ n = 16） and a group of the dead（ n = 12 ）. A healthy control group （ n = 20） was also estaldished. The correlated cytokines were examined by means of LuminexTM-100. Results The activation of NF-κB and levels of IL-6 and IL-10 in children with MODS were higher than those in control group （ P 〈 0. 05 ） ,and they were consistently higher in the dead group than in the recovered group （ P 〈0. 05） ,while the level of TNF-α decreased more significantly in MODS group than in control group. The increased activity of NF-κB was positively correlated with the level of IL-6 （ r = 0. 292, P = 0. 007 ） , but negatively correlated with the level of TNF-α （ r = 0. 333, P = 0. 002 ）. Conclusion Persistent elevation of NF-κB activation and IL-6 ,IL-10 levels correlates with poor outcome in children with MODS.

关 键 词：多器官功能障碍综合征 核因子-ΚB 炎性因子 

分 类 号：R72[医药卫生—儿科]