白介素-13与γ-干扰素在急性毛细支气管炎患儿血清中的表达及其临床意义  

Serum levels of interleukin-13 and interferon-γ in infants with acute bronchiolitis and its clinical significance

作　　者：张义[1] 范丽[1] 周华[1] 

机构地区：[1]遵义医学院附属医院儿科,贵州遵义563003

出　　处：《中国实用儿科杂志》2007年第12期914-915,共2页Chinese Journal of Practical Pediatrics

摘　　要：目的探讨白介素-13(IL-13)、γ-干扰素(IFN-γ)在急性期毛细支气管炎患儿血清中的表达及与病情轻重之间的相关性。方法用酶联免疫吸附(ELISA)法测定2005-02—2005-11于遵义医学院附属医院治疗的42例急性期毛细支气管炎患儿(其中轻症组22例,重症组20例)和16名健康婴儿的血清IL-13、IFN-γ的质量浓度。结果急性期毛细支气管炎患儿血清IL-13的质量浓度[(6.88±2.12)ng/L]明显高于对照组[(5.48±1.28)ng/L,P<0.01]。急性期毛细支气管炎患儿IFN-γ质量浓度[(10.71±2.44)ng/L]明显高于对照组[(9.20±1.54)ng/L,P<0.05];其中轻症组明显高于重症组和对照组(P<0.05),而重症组与对照组差异则无显著性(P>0.05)。结论急性期毛细支气管炎患儿血清IL-13质量浓度明显增高,提示IL-13参与了毛细支气管炎的发病过程,但所测质量浓度不能反映病情严重程度;IFN-γ在轻症毛细支气管炎组质量浓度明显增高,而重症组不增高,这可能与急性重症毛细支气管炎患儿IFN-γ产生受抑制有关。Objective To determined the serum levels of interleukin-13 （ IL-13 ） and interferon-γ（ IFN-γ） in infants with acute bmnehiolitis. Methods The serum levels of IL-13 and IFN-γ of 42 cases with acute bronchiolitis and 16 cases of control were determined with enzyme linked immunosorbent assay （ ELISA）. Results The serum levels of IL-13 in inrants with acute brnnehinlitis [ （ 6. 88± 2. 12 ） ng/L] were significantly higher than those in the 16 healthy infants （the control） [（5.48 ± 1.28） ng/L] （ P 〈0. 01 ）. The serum levels of IFN-γ in infants with acute bronehiolitis [ （10.71 ± 2. 44） ng/L ] were significantly higher than those in the control [ （ 9. 20 ± 1.54） ng/L ] { P 〈 0. 05 ）. The serum level of IFN-γ in infants with mild bmnehiolitis was significantly higher than those with severe bmnehiolitis and the control. There was no difference in serum levels of IFN-γ between the cases with severe bmnchiolitis and the control （ P 〉 0. 05 ）. Condusion This study provids the evidence that serum levels of IL-13 are increased in cases with acute bmnchiolitis,indiearing the IL-13 may play a role in the pathogenesis of acute bmnehiolitis.

关 键 词：毛细支气管炎 白介素-13 Γ-干扰素 

分 类 号：R72[医药卫生—儿科]