萎康胶囊对胃癌前期病变大鼠胃黏膜凋亡相关蛋白表达的影响  

Effect of Weikang Capsules on expression of apoptosis-related proteins of gastric mucosa in rats with chronic atrophic gastritis of precancerous lesion

作　　者：韩哲[1] 李军祥[1] 田德禄[2] 鲁香凤[3] 戴欣[3] 

机构地区：[1]北京中医药大学东方医院消化内科,北京100078 [2]北京中医药大学东直门医院消化内科 [3]北京中医药大学东直门医院病理科

出　　处：《北京中医药大学学报》2007年第11期765-768,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京中医药大学新药研究基金项目

摘　　要：目的从细胞凋亡相关蛋白表达的角度探讨胃癌前期病变的发病机制及萎康胶囊对其治疗的作用机制。方法采用复合方法复制大鼠胃癌前期病变模型,用免疫组化法检测萎康胶囊对胃癌前期病变大鼠胃黏膜上皮细胞凋亡相关蛋白Bcl-2、Bax表达的影响。结果大鼠癌前期病变胃黏膜Bcl-2蛋白阳性表达细胞较正常组大鼠明显增多,表达强度显著增强(P<0.01),而萎康小剂量组和大剂量组Bcl-2表达强度均低于自然恢复组(P<0.01)。正常大鼠胃窦部黏膜腺体增值带较多细胞Bax蛋白呈阳性表达,模型组和自然恢复组,胃癌前期病变区阳性细胞显著减少(P<0.01),尤其在异型增生区域信号强度明显减弱,且随病变程度加重表达减弱。萎康小剂量组和大剂量组Bax表达强度均高于自然恢复组(P<0.01)。结论胃癌前期病变大鼠胃黏膜上皮细胞存在着Bcl-2、Bax蛋白表达的异常现象,萎康胶囊则有下调胃癌前期病变大鼠胃黏膜Bcl-2蛋白的表达水平和上调Bax蛋白的表达水平从而发挥治疗胃癌前期病变的作用。Objective To investigate the pathogenesis of chronic atrophic gastritis of precancerous lesion and therapeutic mechanism of Weikang Capsules on the base of expression of apoptosis-related proteins. Methods The model of chronic atrophic gastritis of precancerous lesion was established by using a synthetic method. The effect of Weikang Capsules on expression of apoptosis-related proteins Bcl-2 and Bax of gastric mucosa in rats with chronic atrophic gastritis of precancerous lesion was observed by using immunohistochemistry method. Results In the gastric mucosa in rats with chronic atrophic gastritis of precancerous lesion, the positive expression of Bcl-2 protein was increased significantly than that in the normal group（P 〈0.01 ）,while decreased in the low-dose and high-dose of Weikang Capsules groups with a significant difference compared with that of the spontaneous recovery group （ P 〈 O. O1 ）. The positive expression of Bax protein was increased in normal group, while significantly decreased in the precancerous lesion of those model group and spontaneous recovery group（ P 〈 O. O1 ）. Especially in thedysplasia area, the expression distinctively decreased in degrees with the development of dysplasia. The positive expression of Bax protein was increased in the low-dose and high-dose of Weikang Capsules groups compared with the spontaneous recovery group （P 〈 0. 01 ）. Conclusion The abnormal expressions of Bcl-2 and Bax proterin exist in the epithelial cell of gastric mucosa in rats with chronic atrophic gastritis of precancerous lesion. It suggests that up-regulated expression of Bcl-2 protein and down-regulated expression of Bax protein maybe the mechanism of Weikang Capsules on curing chronic atrophic gastritis of precancerous lesion.

关 键 词：萎康胶囊 胃黏膜 胃癌前期病变 凋亡相关蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]